Kinetic studies on biliverdin-IXalpha reductase
Citation:
Edward Franklin, 'Kinetic studies on biliverdin-IXalpha reductase', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006, pp 331Download Item:
Abstract:
The species distribution of biliverdin-IXa reductase (BVR-A) has been reinvestigated. It
appears increasingly likely that birds and amphibia have the potential to express an active
biliverdin-IXa reductase. The full length cDNAs encoding human, Danio rerio, murine,
and Xenopus BVR-A were successfully amplified and cloned into various expression
vectors. To date human BVR-A pET41a-Mod, murine BVR-A ppMAL, and Xenopus
BVR-A pGEX-KG transformed cultures have been induced with IPTG resulting in the
expression of recombinant proteins which display the appropriate molecular weights. The
cloning and expression of an active Xenopus BVR-A in this study and the detection of
biliverdin reductase activity in turkey erythrocyte cytosol extends the phylogenetic
distribution of BVR-A to include amphibians and possibly birds.
Author: Franklin, Edward
Advisor:
Mantle, TimQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). School of Biochemistry and ImmunologyNote:
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Full text availableKeywords:
Biochemistry, Ph.D., Ph.D. Trinity College DublinMetadata
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