Browsing Biochemistry (Theses and Dissertations) by Date of Publication
Now showing items 1-20 of 289
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Modulation of the inflammatory response by taurine and its structural analogue, HEPES
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Activated neutrophils (PMNs) have been implicated in the production of tissue injury in inflammatory-bowel disease (IBD) through release of proinflammatory mediaters during respiratory burst activity. Taurine, the most ... -
A biochemical oscillator : experimental and theoretical studies of the peroxidase-oxidase reaction
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)The peroxidase-oxidase (PO) reaction is the haem-peroxidase catalysed reaction of molecular oxygen with NADH, and has been shown previously to behave in an oscillatory fashion. It has been used here as a useful laboratory ... -
The development of fish epithelial cell lines and primary cultures as diagnostic tools in environmental risk assessment
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)The objective of the this study was to attempt and develop fish epithelial cellular markers as diagnostic tools for effects assessment in ecotoxicolgy using established cell lines and primary cultures. As a target group ... -
Biochemical investigations of hyperhomocysteinemia
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Hyperhomocysteinemia, a condition where plasma homocysteine occurs at an abnormally elevated level, is a common finding in vascular disease subjects. Several genetic and nutritional factors have been implicated. The first ... -
Semicarbazide-sensitive amine oxidase & 5-HT in dental pulp
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)This thesis investigated the behavior of semicarbazide sensitive amine oxidase (SSAO) and the unique activity that it possesses towards 5-hydroxytryptamine (5-HT) in various dental pulp tissues. This summary presents the ... -
Delayed cell death in human and fish skin cell lines after exposure to UVA and UVB
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)The effects of ultraviolet radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UVA and UVB. This study compares the effects of UVA ... -
The role of amines in paraganglioma, neuroblastoma and carcinoid diagnosis
(Trinity College (Dublin, Ireland). Department of Chemical Pathology, 2000)The role of urinary noradrenaline, adrenaline, dopamine, 4-hydroxy-3-methoxy-mandelic acid (HMMA) and homovanillic acid (HVA) in the diagnosis of paraganglioma especially phaeochromocytoma and in neuroblastoma is investigated. ... -
Differential patterns of gene expression associated with metastasis in colon cancer
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Metastatic spread is the main cause of colon cancer related death and therefore a greater understanding of this process is a prerequisite for improved treatment. The acquisition of metastatic properties confers tumour cells ... -
Mutational and phenotypic analysis of hyperphenylalaninaemia in Southern Ireland
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Phenylketonuria (PKU) is the most common inherited defect of amino acid metabolism in the Irish population occurring at a frequency of 1/4,500 live births. It is caused by a deficiency of the hepatic enzyme Phenylalanine ... -
Differential patterns of gene expression associated with microsatellite instability and chromosomal instability in colorectal cancer
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Two distinct pathways exist in sporadic colorectal cancer. The microsatellite instability pathway (MIN) is recognised by widespread instability caused by aberrant mismatch repair machinery. This genotype accounts for 15% ... -
Investigating the role of Small G Proteins in the activation of p38 MAPK by Interleukin-1
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)This study is an investigation into the role of small G proteins in IL-1 signalling. Lethal Toxin, from Clostridium sordellii, which specifically glucosylates and thereby inactivates the low molecular weight G proteins ... -
Investigation into the mechanism by which a novel series of pyrrolo-1,5-benzoxazepine compounds induce apoptosis in chronic myelogenous leukaemia cells
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Some members of a novel series of pyrrolo-1,5-benzoxazepine compounds induce apoptosis in human chronic myeloid leukaemia (CML) cells. A representative compound, PBOX-6, induces apoptosis in a dose- and time-dependent ... -
Bacterial products as activators of NF-ϰB
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)The ability of bacterial products to potently activate NF-xB has made this transcription factor one of the most widely studied in the immune response. A greater understanding of the mechanisms and signal transduction ... -
Involvement of the low molecular weight G protein Rac1 in IL-1 signalling to NFkB activation
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)The involvement of the low molecular weight G protein Rac1 in IL-1 signal transduction has been suggested by studies that have demonstrated that a dominant negative mutant of Rac1 can inhibit IL-1-induced activation of ... -
The alcohol dehydrogenases of Bacillus stearothermophilus
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)A 1.5-kb fragment of Bacillus stearothermophilus DSM 2334 DNA was subcloned into pUC18, and the nucleotide sequence was determined. The fragment contained a complete open reading frame that encoded a 339-residue amino-acid ... -
The role of thiols in the activation of the platelet integrin αllbβ3
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Platelets play a vital role in maintaining normal haemostasis. They circulate passively in the blood in an non-adhesive slate as discrete smooth discs. When platelets encounter a vessel wall injury or are exposed to foreign ... -
Is Apolipoprotein-E modulated in peripheral blood mononuclear cells by IFN-β and is this to related patient responsiveness in multiple sclerosis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Multiple Sclerosis is a neurodegenerative disorder involving the attack of blood resident T-lymphocytes on the myelin sheath surrounding the neuron. This condition is particularly prevalent amongst white, northern European ... -
Studies on human biliverdin-IX alpha reductase and linear tetrapyrrole signaling
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Human Biliverdin-IXa reductase (BVR-A) has been cloned and overexpressed in E.coli as a GST- and Hexahistidine fusion protein. The full length cDNA encoding the enzyme has been amplified via PCR and hgated into the pGEX-KG ... -
The establishment of hybrid cell lines from human pancreas
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)The need for research into diabetes mellitus is ever increasing. Of specific interest are the mechanisms responsible for glucose-responsive insulin secretion and the autoimmune destruction of pancreatic p-cells. However, ... -
Studies on muscle residues of the anti-bacterial veterinary medicines tilmicosin, enrofloxacin and tiamulin in livestock and the risk to consumers from ingestion of injection-sites
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Studies on incurred residues of the veterinary anti-bacterials tilmicosin, enrofloxacin and tiamulin were undertaken in cattle, sheep and pigs in order to investigate the effects of cooking on injection-site residues and ...