Role for Retinoic Acid-Related Orphan Receptor Alpha (RORα) Expressing Macrophages in Diet-Induced Obesity
Citation:
Hams, E., Roberts, J., Bermingham, R., Hogan, A.E., O’Shea, D., O’Neill, L., Fallon, P.G., Role for RetinoicAcid-Related Orphan Receptor Alpha(RORα) Expressing Macrophages inDiet-Induced Obesity, Frontiers in Immunology, 2020, 11:1966Download Item:
Abstract:
The transcription factor RORα plays an important role in regulating circadian rhythm, inflammation, metabolism, and cellular development. Herein we show a role for RORα-expressing macrophages in the adipose tissue in altering the metabolic state of mice on a high-fat diet. The expression of Rora and RORA is elevated in white adipose tissue from obese mice and humans when compared to lean counterparts. When fed a high-fat diet Rora reporter mice revealed increased expression of Rora-YFP in macrophages in white adipose tissue deposits. To further define the potential role for Rora-expressing macrophages in the generation of an aberrant metabolic state Rorafl/flLysMCre/+ mice, which do not express Rora in myeloid cells, were maintained on a high-fat diet, and metabolic parameters assessed. These mice had significantly impaired weight gain and improved metabolic parameters in comparison to Rorafl/fl control mice. Further analysis of the immune cell populations within white adipose tissue deposits demonstrates a decrease in inflammatory adipose tissue macrophages (ATM). In obese reporter mouse there was increased in Rora-YFP expressing ATM in adipose tissue. Analysis of peritoneal macrophage populations demonstrates that within the peritoneal cavity Rora-expression is limited to myeloid-derived macrophages, suggesting a novel role for RORα in macrophage development and activation, which can impact on metabolism, and inflammation.
Sponsor
Grant Number
Science Foundation Ireland
10/IN.1/B3004
Science Foundation Ireland
15/SIRG/3473
Author's Homepage:
http://people.tcd.ie/laoneill
Author: O'Neill, Luke
Type of material:
Journal ArticleCollections
Series/Report no:
Frontiers in Immunology;11;
1966;
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Full text availableKeywords:
RORα, Macrophage, Inflammation, Metabolism, ObesityDOI:
http://dx.doi.org/10.3389/fimmu.2020.01966Metadata
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