RSS 1.0Biochemistry (Scholarly Publications)
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The MyD88+ Phenotype Is an Adverse Prognostic Factor in Epithelial Ovarian Cancer.
(2014)The prognosis of epithelial ovarian cancer is poor in part due to the high frequency of chemoresistance. Recent evidence points to the Toll-like receptor-4 (TLR4), and particularly its adaptor protein MyD88, as one potential ... -
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
(2012)An important route to understanding how proteins function at a mechanistic level is to have the structure of the target protein available, ideally at atomic resolution. Presently, there is only one way to capture such ... -
Crystal structure of the integral membrane diacylglycerol kinase
(2013)Diacylglycerol kinase catalyses the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid for use in shuttling water-soluble components to membrane-derived oligosaccharide and lipopolysaccharide in the cell ... -
A fast, simple and robust protocol for growing crystals in the lipidic cubic phase
(2012)A simple and inexpensive protocol for producing crystals in the sticky and viscous mesophase used for membrane protein crystallization by the in meso method is described. It provides crystals that appear within 15–30 min ... -
Membrane protein structure determination using crystallography and lipidic mesophases: recent advances and successes.
(2012)The crystal structure of the β 2 -adrenergic receptor in complex with an agonist and its cognate G protein has just recently been solved. It is now possible to explore in molecular detail the means by which this ... -
Crystallizing membrane proteins for structure-function studies using lipidic mesophases.
(2011)The lipidic cubic phase method for crystallizing membrane proteins has posted some high-profile successes recently. This is especially true in the area of G-protein-coupled receptors, with six new crystallographic ... -
Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes.
(2011)The lipidic cubic mesophase has been used to crystallize important membrane proteins for high-resolution structure determination. To date, however, no integral membrane enzymes have yielded to this method, the in meso. For ... -
Membrane Protein Crystallization in Lipidic Mesophases. Hosting lipid affects on the crystallization and structure of a transmembrane peptide.
(2011)Gramicidin is an apolar pentadecapeptide antibiotic consisting of alternating D-and L-amino acids. It functions, in part, by creating pores in membranes of susceptible cells rendering them leaky to monovalent cations. ... -
Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen
(2011)The default lipid for the bulk of the crystallogenesis studies performed to date using the cubic mesophase method is monoolein. There is no good reason however, why this 18-carbon, cis - monounsaturated monoacylglycerol ... -
Crystallizing membrane proteins for structure determination using lipidic mesophases.
(2010)A detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be ... -
Identification and Characterization of a Stage Specific Membrane Protein Involved in Flagellar Attachment in Trypanosoma brucei
(2013)Flagellar attachment is a visibly striking morphological feature of African trypanosomes but little is known about the requirements for attachment at a molecular level. This study characterizes a previously undescribed ... -
Nutrient ingestion increased mTOR signaling, but not hVps34 activity in human skeletal muscle after sprint exercise.
(2013)Nutrient provision after sprint exercise enhances mammalian target of rapa- mycin (mTOR) signaling. One suggested that nutrient sensor is the class III phosphatidylinositol 3-kinase, vacuolar protein sorting 34 (Vps34), ... -
Circadian Clock Proteins and Immunity
(2014)Immune parameters change with time of day and disruption of circadian rhythms has been linked to inflammatory pathologies. A circadian-clock-controlled immune system might allow an organism to anticipate daily changes in ... -
The role of Ets2 transcription factor in the induction of microRNA-155 by LPS, and its targeting by IL-10.
(2014)MicroRNA-155 (miR-155) is highly expressed in many cancers such as B cell lymphomas and myeloid leukaemia, and inflammatory disorders such as rheumatoid arthritis, atopic dermatitis and multiple sclerosis. The role ... -
Variation in Both IL28B and KIR2DS3 Genes Influence Pegylated Interferon and Ribavirin Hepatitis C Treatment Outcome in HIV-1 Co-Infection
(2013)Pegylated-IFN and ribavirin remains the current treatment for chronic HCV infection in patients co-infected with HIV-1, but this regimen has low efficacy rates, particularly for HCV genotype 1/4 infection, has severe side ... -
Poxviral protein A46 antagonizes Toll-like receptor 4 signaling by targeting BB loop motifs in Toll-IL-1 receptor adaptor proteins to disrupt receptor:adaptor interactions.
(2012)TLR4 signaling is inhibited by poxviral protein A46, but the mechanism is unknown. Results: We identify the protein interaction surfaces within the TLR4 complex that A46 antagonises, and characterize the interaction between ... -
HUMAN INTERLEUKIN-1 RECEPTOR ASSOCIATED KINASE-2 (IRAK-2) IS ESSENTIAL FOR TOLL-LIKE RECEPTOR-MEDIATED TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL REGULATION OF TNFα
(2011)Toll-like receptors (TLRs) are pattern recognition receptors that recognise microbial ligands and subsequently trigger intracellular signalling pathways involving transcription factors such as NF?B and MAPKs such ... -
Innate sensing of bacterial cyclic dinucleotides: More than just STING
(2012)Bacterial cyclic dinucleotides are recognised by the innate immune system, leading to induction of type I interferons. The mammalian helicase DDX41 directly binds cyclic dinucleotides and mediates the signaling pathway ...