Structure-function and pharmacological studies on the mammalian serotonin transporter
Citation:
Siobhán Mary Scanlon, 'Structure-function and pharmacological studies on the mammalian serotonin transporter', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002, pp 447Abstract:
In Pichia pastoris, the existing membrane ergosterol was replaced by cholesterol in an attempt to improve folding of the low affinity heterologously expressed rat serotonin transporter, as judged by means of radioligand binding of [3H]imipramine. Two methods were used to incorporate cholesterol into the Pichia membranes. Firstly, an in vivo approach was used, which exploited metabolic inhibitors to disrupt the ergosterol
biosynthetic pathway and induce a requirement for exogenously supplied cholesterol. Two compounds, lovastatin and pravastatin, were tested for their potency at inhibiting ergosterol production.
Author: Scanlon, Siobhán Mary
Advisor:
Williams, CliveQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). School of Biochemistry and ImmunologyNote:
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Full text availableKeywords:
Biochemistry, Ph.D., Ph.D. Trinity College DublinMetadata
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