Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus.
Item Type:Journal Article
Citation:Cherezov V, Hofer N, Szebenyi DM, Kolaj O, Wall JG, Gillilan R, Srinivasan V, Jaroniec CP, Caffrey M, Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus., Structure (London, England : 1993), 16, 9, 2008, 1378-88
Insights into the Mode of Action of a Putative Zinc Transporter CzrB in Thermus.pdf (Published (publisher's copy) - Peer Reviewed) 3.772Mb
The crystal structures of the cytoplasmic domain of the putative zinc transporter CzrB in the apo and zinc-bound forms reported herein are consistent with the protein functioning in vivo as a homodimer. NMR, X-ray scattering, and size-exclusion chromatography provide support for dimer formation. Full-length variants of CzrB in the apo and zinc-loaded states were generated by homology modeling with the Zn2+/H+ antiporter YiiP. The model suggests a way in which zinc binding to the cytoplasmic fragment creates a docking site to which a metallochaperone can bind for delivery and transport of its zinc cargo. Because the cytoplasmic domain may exist in the cell as an independent, soluble protein, a proposal is advanced that it functions as a metallochaperone and that it regulates the zinc-transporting activity of the full-length protein. The latter requires that zinc binding becomes uncoupled from the creation of a metallochaperone-docking site on CzrB.
Science Foundation Ireland
Author: CAFFREY, MARTIN
Type of material:Journal Article
Series/Report no:Structure (London, England : 1993)
Availability:Full text available