Show simple item record

dc.contributor.authorCAFFREY, MARTINen
dc.date.accessioned2010-04-27T11:30:43Z
dc.date.available2010-04-27T11:30:43Z
dc.date.issued2008en
dc.date.submitted2008en
dc.identifier.citationCherezov V, Hofer N, Szebenyi DM, Kolaj O, Wall JG, Gillilan R, Srinivasan V, Jaroniec CP, Caffrey M, Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus., Structure (London, England : 1993), 16, 9, 2008, 1378-88en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/39194
dc.descriptionPUBLISHEDen
dc.description.abstractThe crystal structures of the cytoplasmic domain of the putative zinc transporter CzrB in the apo and zinc-bound forms reported herein are consistent with the protein functioning in vivo as a homodimer. NMR, X-ray scattering, and size-exclusion chromatography provide support for dimer formation. Full-length variants of CzrB in the apo and zinc-loaded states were generated by homology modeling with the Zn2+/H+ antiporter YiiP. The model suggests a way in which zinc binding to the cytoplasmic fragment creates a docking site to which a metallochaperone can bind for delivery and transport of its zinc cargo. Because the cytoplasmic domain may exist in the cell as an independent, soluble protein, a proposal is advanced that it functions as a metallochaperone and that it regulates the zinc-transporting activity of the full-length protein. The latter requires that zinc binding becomes uncoupled from the creation of a metallochaperone-docking site on CzrB.en
dc.format.extent1378-88en
dc.language.isoenen
dc.relation.ispartofseriesStructure (London, England : 1993)en
dc.relation.ispartofseries16en
dc.relation.ispartofseries9en
dc.rightsYen
dc.subjectproteinen
dc.titleInsights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus.en
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorEnterprise Irelanden
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcaffreen
dc.identifier.rssinternalid65885en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record