Camelpox virus encodes a schlafen-like protein that affects orthopoxvirus
Citation:
Gubster C, Goodbody R, Ecker A, Brady G, O'Neill LA, Jacobs N, Smith GL., Camelpox virus encodes a schlafen-like protein that affects orthopoxvirus, Journal of General Virology, 88, 6, 2007, 1667-1676Download Item:
Abstract:
Camelpox virus (CMLV) gene 176R encodes a protein with sequence similarity to murine schlafen (m-slfn) proteins. In vivo, short and long members of the m-slfn family inhibited T-cell development, whereas in vitro, only short m-slfns caused arrest of fibroblast growth. CMLV 176 protein (v-slfn) is most closely related to short m-slfns; however, when expressed stably in mammalian cells, v-slfn did not inhibit cell growth. v-slfn is a predominantly cytoplasmic 57 kDa protein that is expressed throughout infection. Several other orthopoxviruses encode v-slfn proteins, but the v-slfn gene is fragmented in all sequenced variola virus and vaccinia virus (VACV) strains. Consistent with this, all 16 VACV strains tested do not express a v-slfn detected by polyclonal serum raised against the CMLV protein. In the absence of a small animal model to study CMLV pathogenesis, the contribution of CMLV v-slfn to orthopoxvirus virulence was studied via its expression in an attenuated strain of VACV. Recombinant viruses expressing wild-type v-slfn or v-slfn tagged at its C terminus with a haemagglutinin (HA) epitope were less virulent than control viruses. However, a virus expressing v-slfn tagged with the HA epitope at its N terminus had similar virulence to controls, implying that the N terminus has an important function. A greater recruitment of lymphocytes into infected lung tissue was observed in the presence of wild-type v-slfn but, interestingly, these cells were less activated. Thus, v-slfn is an orthopoxvirus virulence factor that affects the host immune response to infection.
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http://people.tcd.ie/laoneillhttp://people.tcd.ie/bradyg1
Description:
PUBLISHEDPMID: 17485525
Author: O'NEILL, LUKE; BRADY, GARETH
Type of material:
Journal ArticleCollections
Series/Report no:
Journal of General Virology88
6
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Full text availableKeywords:
BiochemistrySubject (TCD):
Immunology, Inflammation & Infection , NeuroscienceDOI:
http://dx.doi.org/10.1099/vir.0.82748-0Metadata
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