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dc.contributor.authorO'NEILL, LUKEen
dc.contributor.authorBRADY, GARETHen
dc.date.accessioned2009-09-29T17:03:06Z
dc.date.available2009-09-29T17:03:06Z
dc.date.issued2007en
dc.date.submitted2007en
dc.identifier.citationGubster C, Goodbody R, Ecker A, Brady G, O'Neill LA, Jacobs N, Smith GL., Camelpox virus encodes a schlafen-like protein that affects orthopoxvirus, Journal of General Virology, 88, 6, 2007, 1667-1676en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/33437
dc.descriptionPUBLISHEDen
dc.descriptionPMID: 17485525en
dc.description.abstractCamelpox virus (CMLV) gene 176R encodes a protein with sequence similarity to murine schlafen (m-slfn) proteins. In vivo, short and long members of the m-slfn family inhibited T-cell development, whereas in vitro, only short m-slfns caused arrest of fibroblast growth. CMLV 176 protein (v-slfn) is most closely related to short m-slfns; however, when expressed stably in mammalian cells, v-slfn did not inhibit cell growth. v-slfn is a predominantly cytoplasmic 57 kDa protein that is expressed throughout infection. Several other orthopoxviruses encode v-slfn proteins, but the v-slfn gene is fragmented in all sequenced variola virus and vaccinia virus (VACV) strains. Consistent with this, all 16 VACV strains tested do not express a v-slfn detected by polyclonal serum raised against the CMLV protein. In the absence of a small animal model to study CMLV pathogenesis, the contribution of CMLV v-slfn to orthopoxvirus virulence was studied via its expression in an attenuated strain of VACV. Recombinant viruses expressing wild-type v-slfn or v-slfn tagged at its C terminus with a haemagglutinin (HA) epitope were less virulent than control viruses. However, a virus expressing v-slfn tagged with the HA epitope at its N terminus had similar virulence to controls, implying that the N terminus has an important function. A greater recruitment of lymphocytes into infected lung tissue was observed in the presence of wild-type v-slfn but, interestingly, these cells were less activated. Thus, v-slfn is an orthopoxvirus virulence factor that affects the host immune response to infection.en
dc.description.sponsorshipThis work was supported by grants from the Wellcome Trust. G. L. S. is a Wellcome Trust Principal Research Fellow.en
dc.format.extent1667-1676en
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.relation.ispartofseriesJournal of General Virologyen
dc.relation.ispartofseries88en
dc.relation.ispartofseries6en
dc.rightsYen
dc.subjectBiochemistryen
dc.titleCamelpox virus encodes a schlafen-like protein that affects orthopoxvirusen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/laoneillen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/bradyg1en
dc.identifier.rssinternalid50645en
dc.identifier.doihttp://dx.doi.org/10.1099/vir.0.82748-0en
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDThemeNeuroscienceen
dc.identifier.rssurihttp://dx.doi.org/10.1099/vir.0.82748-0
dc.status.accessibleNen


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