Browsing Microbiology (Theses and Dissertations) by Date of Publication
Now showing items 1-20 of 82
-
Analysis of the chloramphenicol resistance determinant of plasmid R26
(Trinity College (Dublin, Ireland). Department of Microbiology, 1985)The Inc P-1 plasmid, R2B carries an inducible chloramphenicol resistance determinant, cml. This specifies low level chloramphenicol resistance (30 μg/ml] in Escherichia coli. The mechanism does not involve drug detoxification ... -
Helicobacter pylori infection : in vitro diagnostic methods, antimicrobial susceptibility testing, epidemiology of recrudescence and clarithromycin resistance
(Trinity College (Dublin, Ireland). Department of Microbiology, 2000)H. pylori is a Gram-negative, non-spore forming, curved bacterium that colonises the gastric epithelium of the human stomach. H. pylori is firmly established as an aetiological agent in peptic ulcer disease and successful ... -
Interaction of heat shock proteins with splicing machinery components during and after thermal stress in Saccharomyces cerevisiae
(Trinity College (Dublin, Ireland). Department of Microbiology, 2000)This thesis is a study of the amehorative effects of Heat Shock Proteins (Hsps) on the cellular process of mRNA splicing during and after thermal stress in Saccharomyces cerevisiae. Splicing is a vital process that is known ... -
Analysis of the role of human papillomaviruses and the p53/Rb tumour suppressor genes in the development of cutaneous squamous cell carcinoma
(Trinity College (Dublin, Ireland). Department of Microbiology, 2000)Nonmelanoma skin cancers (NMSC) are the most frequent cancers in Caucasian populations. Renal transplant recipients (RTR) are at an increased risk of neoplasia, particularly skin cancer. Studies on anogenital cancer have ... -
Recombinant vaccines against infectious pancreatic necrosis virus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2000)The rapid expansion of the aquaculture industry in recent years has led to an increase in disease outbreaks. Over the last few years bacterial disease problems have been brought under control, largely due to the use of a ... -
Molecular characterisation of agn43 and its encoded protein, antigen 43, the major phase-variable outer membrane protein of Escherichia coli
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)A number of year ago workers in this laboratory identified antigen 43 (Ag43) during a systematic immunochemical analysis of the envelopes of Escherichia coli ML308-225 O13:O68:H . This Escherichia-specific outer membrane ... -
Variations in silver stained nucleolar organiser regions(AgNORs) in normal, irradiated and neoplastic tissues
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)Nucleolar organiser regions (NORs) are loops of ribosomal DNA which contain the genes that transcribe to ribosomal RNA. They are situated on the short arm of the acrocentric human chromosomes 13, 14, 15, 21 and 22. Several ... -
Characterisation of SdrC, SdrD and SdrE from the serine-aspartate repeat family of Staphylococcus auerus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)Staphylococcus aureus is an important human pathogen. The ability of S. aureus to initiate infection is attributed at least partly to the expression of cell wall-associated proteins known as MSCRAMMs (microbial surface ... -
Investigations into the pathogenesis of Dengue virus infections of humans : the endothelium as a target
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)The pathogenesis of the disease Dengue Haemorrhagic Fever/Dengue Shock Syndrome has not been elucidated. This study investigated the effects of Dengue virus, serotype 2 infection on both the human cell line ECV304/T-24 ... -
Characterisation of genotypic and phenotypic differences between Candida dubliniensis and Candida albicans
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)Summary -Candida dubliniensis is a recently described Candida species associated with oral candidosis in Human Immunodeficiency Virus (HIV)-infected and AIDS patients. The phylogenetic position of C. dubliniensis has ... -
Microevolution in Helicobacter pylori
(Trinity College (Dublin, Ireland). Department of Microbiology, 2002)Helicobacter pylori chronically colonises the human gastric mucosa. It is a major cause of chronic active gastritis and peptic ulcer disease and is associated with the development of gastric neoplasia. The population ... -
Epidemiology of Streptococcus agalactiae (Group B Streptococci) in Dublin Maternity Hospitals
(Trinity College (Dublin, Ireland). Department of Microbiology, 2002)Streptococcus agalactiae (Group B Streptococci) are Gram-positive encapsulated cocci distinguished from other streptococci by their narrower zones of P-haemolysis. Group B Streptococci (GBS) was first described as an ... -
Genetic diversity of Helicobactor pylori isolates through Microevolution in vivo
(Trinity College (Dublin, Ireland). Department of Microbiology, 2004)Chronic infection with H. pylori causes peptic ulcer disease and its presence is strongly linked with gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. One of the most interesting characteristics ... -
Immune responses to cereal prolamin proteins in coeliac disease
(Trinity College (Dublin, Ireland). Department of Microbiology, 2004)Coeliac disease is an inflammatory disease of the small intestine, precipitated in susceptible individuals by gliadin, the alcohol soluble (prolamin) fraction of wheat gluten. There is a strong genetic influence on ... -
Virulence gene regulation by nucleoid associated proteins in Salmonella typhimurium : a role for Fis in Salmonella pathogenicity island 2 gene regulation
(Trinity College (Dublin, Ireland). Department of Microbiology, 2004)Nucleoid associated proteins are involved in many diverse biological functions in S. typhimurium. In addition to their structural role within the genome they have been im plicated in transcriptional regulation of a variety ... -
Molecular analysis of surface proteins of staphylococci
(Trinity College (Dublin, Ireland). Department of Microbiology, 2004)S. lugdunensis is an important human pathogen that causes 44% of coagulase negative staphylococcal native valve endocarditis as well as infections such as osteomyelitis and soft tissue abscesses. S. lugdunensis expresses ... -
Identification and characterisation of an FK506-binding protein from Plasmodium falciparum
(Trinity College (Dublin, Ireland). Department of Microbiology, 2004)Malaria remains one of the most significant diseases worldwide. The most common and severe form of the disease is caused by Plasmodium falciparum. The need for new anti-malarial compounds has intensified in recent years ... -
Gene regulation and the Fis nucleoid-associated protein in Salmonella enterica serovar Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2005)The Fis protein was found to influence the superhelical density of DNA and its ability to do this differed between Salmonella enterica serovar Typhimurium (S. typhimurium) and Escherichia coli (E. coli). Induction of the ... -
Tumour therapy using cytokine-expressing semliki forest virus vectors
(Trinity College (Dublin, Ireland). Department of Microbiology, 2005)Semliki Forest Virus (SFV) vector is a transient RNA based suicidal expression vector system and has been previously used as a potential anti-cancer agent. Recently, a new enhanced SFV vector has been developed, pSFV10-E. ... -
Analysis of the effects of Beta-Amyloid and age on cell signalling in rat hippocampus : effect of treatment with polyunsaturated fatty acids
(Trinity College (Dublin, Ireland). Department of Microbiology, 2005)Long-term potentiation (LTP) is considered to be a possible neurophysiological model for learning and memory and consistent with this, is the observation that LTP is sensitive to stress, injury and insult. This thesis ...
