Investigations into the pathogenesis of Dengue virus infections of humans : the endothelium as a target
Citation:
Tara Anne Kelly, 'Investigations into the pathogenesis of Dengue virus infections of humans : the endothelium as a target', [thesis], Trinity College (Dublin, Ireland). Department of Microbiology, 2001, pp 209Download Item:
Abstract:
The pathogenesis of the disease Dengue Haemorrhagic Fever/Dengue Shock
Syndrome has not been elucidated. This study investigated the effects of Dengue virus,
serotype 2 infection on both the human cell line ECV304/T-24 and primary HUVEC, as
an in vitro endothelial cell model system. Infection of ECV304/T-24 cells by DEN 2 resulted in a loss of viability of the cells and death by apoptosis. However, the cells recovered and were persistently
infected by the virus. Infection was productive, with the release o f infectious viral
progeny into the supernatant and maximum production was found at 48 hours post
infection. Persistently infected cells secreted DEN 2 at lower titres. Viral antigen
expression within infected ECV304/T-24 cells and HUVEC was investigated using
indirect immunofluorescence analysis.
Author: Kelly, Tara Anne
Advisor:
O'Sullivan, AideenQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). Department of MicrobiologyNote:
TARA (Trinity’s Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.iePrint thesis water damaged as a result of the Berkeley Library Podium flood 25/10/2011
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Full text availableKeywords:
Microbiology, Ph.D., Ph.D. Trinity College DublinMetadata
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