Tumour therapy using cytokine-expressing semliki forest virus vectors
Citation:
Gowda C. P. Chikkanna, 'Tumour therapy using cytokine-expressing semliki forest virus vectors', [thesis], Trinity College (Dublin, Ireland). Department of Microbiology, 2005, pp 283Download Item:
Abstract:
Semliki Forest Virus (SFV) vector is a transient RNA based suicidal
expression vector system and has been previously used as a potential anti-cancer
agent. Recently, a new enhanced SFV vector has been developed, pSFV10-E. Cells
transfected with this vector yield up to ten times more foreign protein than cells
transfected with original (non-enhanced) expression vector pSFV10. The two IL-12
gene subunits were cloned from mouse splenocytes and inserted into the pSFV10-E
and pSFV10 vectors. Both the pSFV-mIL-12 constructs were characterised for their
expression levels qualitatively and quantitatively in BHK-21 and K-BALB cells. The
secretion of biologically active mIL-12 was confirmed by inducing splenocytes for
IFN-y production using IL-12 expressed by BHK-21 and K-BALB cells. K-BALB,
CT26, and 4T1 tumours are the experimental tum our models in our study.
Author: Gowda C. P. Chikkanna
Advisor:
Atkins, GregoryQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). Department of MicrobiologyNote:
TARA (Trinity's Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.ieType of material:
thesisAvailability:
Full text availableKeywords:
Microbiology, Ph.D., Ph.D. Trinity College DublinMetadata
Show full item recordLicences: