Browsing Biochemistry (Theses and Dissertations) by Date of Publication
Now showing items 21-40 of 290
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Factors influencing the induction of immune responses with DNA vaccines
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002)Compared with any other medical discovery vaccines have prevented more deaths and suffering. Emergence of novel pathogens accentuates the requisite for ongoing development and improvement of traditional vaccination strategies. ... -
Analysis of the MAPK pathways, in silico and in vitro
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002)This thesis takes a combined computational and experimental approach to study the MAPK pathways. These proteins were chosen as they are highly conserved in both sequence and function across all eukaryotes. An evolutionary ... -
Modulation of innate and adaptive immune responses by intranasally delivered adjuvants
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002)Vaccines have had a significant impact on human health for over a hundred years, resulting in the prevention of many millions of deaths each year from infectious diseases. However in recent decades, the evolution of ... -
Structure-function and pharmacological studies on the mammalian serotonin transporter
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002)In Pichia pastoris, the existing membrane ergosterol was replaced by cholesterol in an attempt to improve folding of the low affinity heterologously expressed rat serotonin transporter, as judged by means of radioligand ... -
Characterisation of signalling pathways activated by the IL-1 receptor homologue T1/ST2
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2003)T1/ST2 is a member of the IL-1 receptor family possessing the three characteristic extracellular Ig domains and an intracellular Toll-IL1R (TIR) domain. It is an orphan receptor expressed on a variety of cell types including ... -
Structural and functional analysis of Interleukin-1 F5, a novel member of the Interleukin-1 family
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2003)This study is an investigation into novel members of the Interleukin-1 (IL-1) family; IL-IF5 and IL-1F6. The classical members of the IL-1 family, including IL -1a , IL-1β and IL-18, are involved in immune responses to ... -
Trafficking and regulation of the serotonin transporter
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)The serotonin transporter (SERT) is responsible for the clearance of serotonin from the synaptic cleft. Together with the norepinephrine transporter and dopamine transporter, SERT is of great interest because it is the ... -
Modulation of immune responses by filamentous haemagglutinin from Bordetella pertussis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)The aim of this study was to investigate the mechanisms by which filamentous haemagglutinin (FHA) from Bordetella pertussis modulates immune responses. FHA is an adhesion molecule of B. pertussis and facilitates bacterial ... -
Immediate-Early-Gene (IEG) expression in excitotoxicity
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)The mouse cerebellar granule cell system (mCGCs; Schousboe et al, 1989) was established in this laboratory (Bayer AG, Germany) as a suitable cell culture model for excitotoxicity studies. Suitability was confirmed by ... -
An investigation into the expression and physiological function of mitochondrial uncoupling proteins
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)Our study provides evidence that rat thymus contains a functioning mitochondrial uncoupling protein (UCP 1). Using reverse transcriptase-polymerase chain reaction (RT-PCR), we showed that rat thymus contains UCP 1 mRNA. ... -
Investigation into the mechanism underlying the antiproliferative and apoptotic effects of pyrrolo-1,5-benzoxazepine compounds
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)A novel series of pyrrolo-1,5-benzoxazepine (PBOX) compounds have potential as anti-cancer agents. The PBOX compounds appear to be subdivided into two groups, those that exert antiproliferative effects (e.g. PBOX-21) by ... -
Local cellular and humoral immunity to Bordetella pertussis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)The development of whole cell vaccine (Pw) and acellular pertussis vaccines (Pa) has had a huge impact on the incidence of whooping cough worldwide. The traditional Pw had high protective efficacy, but were associated with ... -
Factors affecting mitochondrial efficiency
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)Experiments were designed to investigate for the first time in situ synaptosomal mitochondrial bioenergetic function using synaptosomes prepared from the whole brain of rats and to compare bioenergetic functions in ... -
Activation of innate immunity by Bordetella pertussis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Respiratory infection with Bordetella pertussis is associated with the induction of Th1 cells and these cells, as well as antibody and cells of the innate immune system mediate the clearance of bacteria from the lungs. ... -
Modulation of innate and adaptive anti-tumour immune responses
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Cancer is the second leading cause of death in the developed world after cardiovascular disease, with almost 25 million people living with cancer worldwide. The three main therapeutic approaches for the treatment of cancer ... -
Schistosoma mansoni modulation of allergic responses
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)It has been proposed that in humans infection with certain parasitic helminths may reduce the propensity to develop allergies in infected populations. Schistosoma mansoni is a parasitic trematode that is implicated as a ... -
Investigation into folate metabolism and the effects of Methotrexate on folate metabolism in relation to the MTHFR C677T mutation in cultured mammalian cells
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)An English physician in 1920 identified an anaemia seen frequently in late pregnancy which responded well to treatment with a yeast extract called marmite (Wills, 1931). She called this anaemia "pernicious anaemia of late ... -
Mechanisms of immunomodulatory activity of Cholera toxin
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Cholera toxin (CT), a major enterotoxin produced by Vibrio cholerae, is a potent mucosal adjuvant that promotes type 2 T helper cell (Th2) responses to coadministered foreign antigen. However, the mechanisms involved in ... -
A study of the multiple functions and interactions of semicarbazide sensitive amine oxidase
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)This thesis focuses on three of the recognised multiple functions of mammalian semicarbazide sensitive amine oxid ase (SSAO) and the contribution of monoamine oxidase (MAO) to some of these. SSAO is a vascular-adhesion ... -
Modulation of Interleukin-1 and Toll-like receptor signalling by the Vaccinia virus protein A52R
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Vaccinia virus (VV) has many m echanisms to suppress and modulate the host im mune response. Given the role of Toll-like receptors (TLRs) and interleukin 1 (IL-1) in immunity, it was possible that VV would have mechanisms ...
