An investigation into the role and regulation of uncoupling protein 1 (UCP 1)
Citation:
Kieran John Clarke, 'An investigation into the role and regulation of uncoupling protein 1 (UCP 1)', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011, pp 248Download Item:
Clarke TCD THESIS 9713 An investigation.pdf (PDF) 104.2Mb
Abstract:
The use of the uncoupling protein inhibitor GDP was used to investigate UCP 1 dependent ROS production in BAT and thymus mitochondria. The effect of UCP 1 inhibition by GDP was then corroborated using UCP 1 ablated mice and their wild-type controls. It was shown that UCP 1 was capable of attenuating ROS production in isolated BAT mitochondria. ROS due to reverse electron transport was shown to be particularly sensitive to UCP 1 attenuation. We have shown for the first time that BAT mitochondria from UCP 1
knock-out mice have significantly less complex I activity and abundance, thereby reducing ROS production due to reverse electron transport, and possibly explaining previous reports of no difference in
oxidative damage in BAT mitochondria from UCP 1 knock-out and wildtype mice. We also show for the first time that BAT mitochondria from UCP 1 knock-out mice, surprisingly, have significantly greater glycerol-
3-phosphate dehydrogenase activity.
Author: Clarke, Kieran John
Advisor:
Porter, RichardQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). School of Biochemistry and ImmunologyNote:
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Biochemistry, Ph.D., Ph.D. Trinity College Dublin.Licences: