Browsing by Subject "Biochemistry, Ph.D."
Now showing items 1-20 of 90
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A biochemical oscillator : experimental and theoretical studies of the peroxidase-oxidase reaction
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)The peroxidase-oxidase (PO) reaction is the haem-peroxidase catalysed reaction of molecular oxygen with NADH, and has been shown previously to behave in an oscillatory fashion. It has been used here as a useful laboratory ... -
A role for type 111 interferons in the natural killer cell immune response to virus
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Natural Killer (NK) cells are fundamental effector cells of the innate immune system that function to eliminate virally infected and transformed cells. One key way in which they do this is through the production of cytokines, ... -
A study of the effects of neurotoxins on the activity and copper of Ceruloplasmin
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)This thesis focuses on the ability of neurotoxins to affect the activity of the copper transport protein ceruloplasmin (EC 1.16.3.1; ferroxidase) and its intrinsic oxidase activities. The protective properties of taurine ... -
Activation of innate immunity by Bordetella pertussis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Respiratory infection with Bordetella pertussis is associated with the induction of Th1 cells and these cells, as well as antibody and cells of the innate immune system mediate the clearance of bacteria from the lungs. ... -
An investigation into the expression and physiological function of mitochondrial uncoupling proteins
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)Our study provides evidence that rat thymus contains a functioning mitochondrial uncoupling protein (UCP 1). Using reverse transcriptase-polymerase chain reaction (RT-PCR), we showed that rat thymus contains UCP 1 mRNA. ... -
An investigation into the physiological expression and function of mitochondrial uncoupling protein 1 in thymus
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Our study confirms the presence of mitochondrial uncoupling protein 1 in thymus. Using laser scanning confocal microscopy, we showed the constitutive expression of UCP 1 in pure thymocytes. Using a primary peptide antibody ... -
An investigation into the role and regulation of uncoupling protein 1 (UCP 1)
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)The use of the uncoupling protein inhibitor GDP was used to investigate UCP 1 dependent ROS production in BAT and thymus mitochondria. The effect of UCP 1 inhibition by GDP was then corroborated using UCP 1 ablated mice ... -
An investigation of the link between the bacterial derived queuine molecule and tyrosine production
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Queuine, a dietary derived 7-deazaguanine molecule, has previously been shown to prevent lethality and other symptoms in germ-free mice that were maintained on a tyrosine-free diet. This work suggested a link between queuine ... -
An investigaton of the innate immune response to the vaccine adjuvant chitosan
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2015)Vaccination is widely regarded as one of the most successful medical intervention strategies to have been introduced. Despite its resounding success, significant challenges in the field of vaccine research still remain. ... -
Analysis of the MAPK pathways, in silico and in vitro
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002)This thesis takes a combined computational and experimental approach to study the MAPK pathways. These proteins were chosen as they are highly conserved in both sequence and function across all eukaryotes. An evolutionary ... -
Bacterial products as activators of NF-ϰB
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)The ability of bacterial products to potently activate NF-xB has made this transcription factor one of the most widely studied in the immune response. A greater understanding of the mechanisms and signal transduction ... -
Biochemical investigations of hyperhomocysteinemia
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Hyperhomocysteinemia, a condition where plasma homocysteine occurs at an abnormally elevated level, is a common finding in vascular disease subjects. Several genetic and nutritional factors have been implicated. The first ... -
Bioenergetics and mitochondrial dynamics in cancer cells and neurons
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Mitochondria in mammalian cells exist as a highly dynamic and interconnected reticulum. This reticular ultrastructure is defined by the opposing forces of fission and fusion, mainly mediated by a group of large GTPases ... -
Biological roles of semicarbazide sensitive amine oxidase
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2009)SSAO is a multifunctional protein that catalyses the oxidative deamination of primary amines, according to the overall reaction RCH2NH2 + O2 + H2O -» RCHO + NH3 + H2O2. In this work, some of the recognised physiological ... -
Characterisation of signalling pathways activated by the IL-1 receptor homologue T1/ST2
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2003)T1/ST2 is a member of the IL-1 receptor family possessing the three characteristic extracellular Ig domains and an intracellular Toll-IL1R (TIR) domain. It is an orphan receptor expressed on a variety of cell types including ... -
Characterisation of the innate immune response to Campylobacter jejuni colonisation in the chicken intestine
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Campylobacter jejuni is the leading cause of gastroenteritis in humans, and chickens are the primary vector for infection. The microbe colonises the caeca of birds as a commensal, and it is hypothesised that intestinal ... -
Characterisation of the novel leucine rich repeat containing protein KIAA0644
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2008)This thesis reports the existence o f a novel leucine rich repeat containing protein annotated as KIAA0644 in the Kazusa DNA Research Institute database (www.Kazusa.or.jp). which was discovered in a study for proteins ... -
Characterisation of the role of Bruton's tyrosine kinase in toll-like receptor signalling
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2007)Toll-like receptors (TLRs) are the primary surveillance system for the detection of pathogens and are crucial to the activation of the host innate response. TLRs contain an extracellular leucine rich repeat region which ... -
Computational approaches to the display and manipulation of metabolic pathways : theory and practice
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2008)Since metabolic pathways constitute a complex system, a mathematical description, even a structural one, must be simplified. Yet, the model must be sufficiently general so that it will encompass the system that is to be ... -
Derivation of dopaminergic neurons from embryonic and induced pluripotent stem cells
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Dopamine (DA) neurons have several fundamental functions in the brain, dysfunction of which has been implicated in neurological and psychiatric disorders, including Parkinson’s disease (PD) and schizophrenia. Embryonic ...
