dc.contributor.advisor | Porter, Richard | |
dc.contributor.author | Clarke, Kieran John | |
dc.date.accessioned | 2019-11-05T14:52:54Z | |
dc.date.available | 2019-11-05T14:52:54Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Kieran John Clarke, 'An investigation into the role and regulation of uncoupling protein 1 (UCP 1)', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011, pp 248 | |
dc.identifier.other | THESIS 9713 | |
dc.identifier.uri | http://hdl.handle.net/2262/90038 | |
dc.description.abstract | The use of the uncoupling protein inhibitor GDP was used to investigate UCP 1 dependent ROS production in BAT and thymus mitochondria. The effect of UCP 1 inhibition by GDP was then corroborated using UCP 1 ablated mice and their wild-type controls. It was shown that UCP 1 was capable of attenuating ROS production in isolated BAT mitochondria. ROS due to reverse electron transport was shown to be particularly sensitive to UCP 1 attenuation. We have shown for the first time that BAT mitochondria from UCP 1
knock-out mice have significantly less complex I activity and abundance, thereby reducing ROS production due to reverse electron transport, and possibly explaining previous reports of no difference in
oxidative damage in BAT mitochondria from UCP 1 knock-out and wildtype mice. We also show for the first time that BAT mitochondria from UCP 1 knock-out mice, surprisingly, have significantly greater glycerol-
3-phosphate dehydrogenase activity. | |
dc.format | 1 volume | |
dc.language.iso | en | |
dc.publisher | Trinity College (Dublin, Ireland). School of Biochemistry and Immunology | |
dc.relation.isversionof | http://stella.catalogue.tcd.ie/iii/encore/record/C__Rb15152610 | |
dc.subject | Biochemistry, Ph.D. | |
dc.subject | Ph.D. Trinity College Dublin. | |
dc.title | An investigation into the role and regulation of uncoupling protein 1 (UCP 1) | |
dc.type | thesis | |
dc.type.supercollection | thesis_dissertations | |
dc.type.supercollection | refereed_publications | |
dc.type.qualificationlevel | Doctoral | |
dc.type.qualificationname | Doctor of Philosophy (Ph.D.) | |
dc.rights.ecaccessrights | openAccess | |
dc.format.extentpagination | pp 248 | |
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