An oral alpha-galactosylceramide adjuvanted Helicobacter pylori vaccine induces protective IL-1R- and IL-17R-dependent Th1 responses
File Type:
PDFItem Type:
Journal ArticleDate:
2019Access:
openAccessCitation:
Longet, S. and Abautret-Daly, A. and Davitt, C.J.H. and McEntee, C.P. and Aversa, V. and Rosa, M. and Coulter, I.S. and Holmgren, J. and Raghavan, S. and Lavelle, E.C., An oral alpha-galactosylceramide adjuvanted Helicobacter pylori vaccine induces protective IL-1R- and IL-17R-dependent Th1 responses, npj Vaccines, 4, 1, 2019Download Item:
s41541-019-0139-z(1).pdf (PDF) 2.177Mb
Abstract:
Helicobacter pylori causes chronic gastric infection that can lead to peptic ulcers and is an identified risk factor for gastric cancer development. Although much effort has been put into the development of a Helicobacter pylori vaccine over the last three decades, none has yet reached clinical application. Specific challenges pertaining to effective H. pylori vaccine development include the lack of proven vaccine-effective antigens and safe mucosal adjuvants to enhance local immune responses as well as the lack of accepted correlates of protection. Herein, we demonstrate that prophylactic intragastric immunisation with a whole-cell killed H. pylori antigen administered together with the non-toxic oral adjuvant α-galactosylceramide (α-GalCer) induced effective immune protection against H. pylori infection in mice, which was of similar magnitude as when using the “gold standard” cholera toxin as adjuvant. We further describe that this α-GalCer-adjuvanted vaccine formulation elicited strong intestinal and systemic Th1 responses as well as significant antigen-specific mucosal and systemic antibody responses. Finally, we report that the protective intestinal Th1 responses induced by α-GalCer are dependent on CD1d, IL-1R as well as IL-17R signalling. In summary, our results show that α-GalCer is a promising adjuvant for inclusion in an oral vaccine against H. pylori infection.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
SFI/12/RC/227
Science Foundation Ireland (SFI)
12/IA/142
Author's Homepage:
http://people.tcd.ie/lavelleehttp://people.tcd.ie/mcentecr
Description:
PUBLISHEDcited By 0
Author: Lavelle, Edward; Mc Entee, Craig
Type of material:
Journal ArticleCollections:
Series/Report no:
npj Vaccines4
1
Availability:
Full text availableKeywords:
Pylori infection, Helicobacter pylori, VaccineDOI:
http://dx.doi.org/10.1038/s41541-019-0139-zLicences: