A fast, simple and robust protocol for growing crystals in the lipidic cubic phase
Citation:
Aherne, M, Lyons, JA, Caffrey, M, A fast, simple and robust protocol for growing crystals in the lipidic cubic phase, Journal of Applied Crystallography, 45, 6, 2012, 1330-1333Download Item:
Abstract:
A simple and inexpensive protocol for producing crystals in the sticky and viscous mesophase used for membrane protein crystallization by the in meso method is described. It provides crystals that appear within 15–30 min of setup at 293 K. The protocol gives the experimenter a convenient way of gaining familiarity and a level of comfort with the lipidic cubic mesophase, which can be daunting as a material when first encountered. Having used the protocol to produce crystals of the test protein, lysozyme, the experimenter can proceed with confidence to apply the method to more valuable membrane (and soluble) protein targets. The glass sandwich plates prepared using this robust protocol can further be used to practice harvesting and snap-cooling of in meso-grown crystals, to explore diffraction data collection with mesophase-embedded crystals, and for an assortment of quality control and calibration applications when used in combination with a crystallization robot.
Sponsor
Grant Number
National Institutes of Health (NIH)
U54GM094599
National Institutes of Health (NIH)
P50GM073210
National Institutes of Health (NIH)
GM75915
European Union Framework Programme 7 (FP7)
CM0902
Science Foundation Ireland (SFI)
07/IN.1/B1836
Author's Homepage:
http://people.tcd.ie/mcaffreDescription:
PUBLISHED
Author: CAFFREY, MARTIN
Type of material:
Journal ArticleCollections
Series/Report no:
Journal of Applied Crystallography45
6
Availability:
Full text availableKeywords:
crystallization, in meso crystallogenesis, lipids, membrane proteins, mesophase, receptorsDOI:
http://dx.doi.org/10.1107/S0021889812037880Metadata
Show full item recordLicences: