Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus
Citation:
Höfer, N., Kolaj, O., Li, H., Cherezov, V., Gillilan, R., Wall, J., Caffrey, M., Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus., Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 63, 8, 2007, 673-677Download Item:
Abstract:
CzrB is a putative zinc transporter from Thermus thermophilus. The protein is
proposed to consist of a hexahelical transmembrane domain with a cytosolic
extramembranal C-terminus. The latter 92-residue fragment may be expressed
free and may function independently of the full-length integral membrane
protein. A 6 His-tagged form of the water-soluble fragment has been
overexpressed in Escherichia coli and diffraction-quality crystals of the tagged
and tag-free variants have been grown. Preliminary X-ray analyses of tag-free
fragment crystals with (2.2 A ? resolution) and without zinc ions (1.7 A ?
resolution) reveal that the former has at least two zinc ions bound per monomer.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
Author's Homepage:
http://people.tcd.ie/mcaffreDescription:
PUBLISHEDPMID: 17671365
Author: CAFFREY, MARTIN
Type of material:
Journal ArticleCollections
Series/Report no:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications63
8
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Full text availableKeywords:
Biophysics, Thermus thermophilus, diffraction analysisSubject (TCD):
Nanoscience & MaterialsDOI:
http://dx.doi.org/10.1107/S1744309107032277Metadata
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