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Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE andbglSloci in Bacillus subtilis
(Elsevier, 2010)
We report the development of a suite of sixintegrative vectors for construction of single copy transcriptional fusions with the gfpmut3, cfp and iyfp reporter genes in B. subtilis. The promoter fusions are constructed using ...
Activation of plasminogen by staphylokinase reduces the severity of Staphylococcus aureus systemic infection
(2010)
Background. Staphylokinase (SAK) is produced by the majority of Staphylococcus aureus strains. It is an extracellular protein that activates the conversion of human plasminogen (plg) to plasmin. The role played by SAK in ...
The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli
(2010)
Mobile genetic elements are widespread in bacteria, where they cause several kinds of mutations. Although their effects are on the whole negative, rare beneficial mutations caused by insertion sequence elements are frequently ...
The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline.
(2010)
Slits and their Roundabout (Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the
vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties. In ...
Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus.
(2010)
Fibronectin-binding proteins A and B are multifunctional LPXTG staphylococcal adhesins, comprising an N-terminal region that binds fibrinogen and elastin, and a C-terminal domain that interacts with fibronectin. The ...
Construction and development of simliki forest virus vectors for transient gene therapy of model tumours in mice
(Trinity College (Dublin, Ireland). Department of Microbiology, 2010)
Cancer is a molecular and genetic disease that results from multiple alterations of genes, including proto-oncogenes and tumour-suppressor genes. Virotherapy is a branch of cancer gene therapy where oncolytic viruses are ...
Analysis of gene evolution and metabolic pathways using the Candida Gene Order Browser.
(BioMed Central, 2010)
Background: Candida species are the most common cause of opportunistic fungal infection worldwide. Recent sequencing efforts have provided a wealth of Candida genomic data. We have developed the Candida Gene Order Browser ...
Investigation of herbicide-based compounds as novel anti-malarial agents
(Trinity College (Dublin, Ireland). Department of Microbiology, 2010)
Plasmodium falciparum causes the most severe form of malaria in humans. Currently, no effective vaccine exists and the emergence of widespread drug resistance in the parasite has further increased the necessity for novel ...
H-NS silences gfp, the Green Fluorescent Protein gene: gfpTCD is a genetically remastered gfp gene with reduced susceptibility to H-NS-mediated transcription silencing and with enhanced translation
(2010)
The bacterial nucleoid-associated protein H-NS, which preferentially targets and silences A+T-rich genes, binds the ubiquitous reporter gene gfp and dramatically reduces local transcription. We have redesigned gfp to reduce ...