Browsing School of Genetics & Microbiology by Title
Now showing items 1-20 of 515
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A bioinformatics approach to (intra-) genome comparisons
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2002)The analysis of large volumes of genomic data generates special computational needs. A Beowulf-type computer cluster was set up for high-performance computing. Improvements over existing tools for the efficient parallelisation of ... -
A leaf-specific phage T7 RNA polymerase-based system for transgene expression in tobacco chloroplasts
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2001)This thesis describes the development of a chloroplast-localised gene expression system based on the phage T7 RNA polymerase. In order to direct T7 RNAP expression in a leaf-specific manner and to target it to the chloroplast, ... -
Accurate Determination of Phenotypic Information from Historic Thoroughbred Horses by Single Base Extension
(PLoS, 2010)Historic DNA data have the potential to identify phenotypic information otherwise invisible in the historical, archaeological and palaeontological record. In order to determine whether a single nucleotide polymorphism ... -
The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline.
(2010)Slits and their Roundabout (Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties. In ... -
Additions, losses, and rearrangements on the evolutionary route from a reconstructed ancestor to the modern Saccharomyces cerevisiae genome.
(PLoS, 2009)Comparative genomics can be used to infer the history of genomic rearrangements that occurred during the evolution of a species. We used the principle of parsimony, applied to aligned synteny blocks from 11 yeast species, ... -
Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa.
(BioMed Central, 2007)Background The role played by microRNAs (miRs) as common regulators in physiologic processes such as development and various disease states was recently highlighted. Retinitis pigmentosa (RP) linked to RHO (which encodes ... -
Altered ribostasis: RNA-protein granules in degenerative disorders
(2013)The molecular processes that contribute to degenerative diseases are not well understood. Recent observations suggest that some degenerative diseases are promoted by the accumulation of nuclear or cytoplasmic RNA-protein ... -
Alternative routes to optimal expression levels: Evolutionary evidence for competitive RNAs and dosage compensation by gene duplication
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Genetics, 2019)Expression evolution and dosage constraints are important factors shaping genomic content and innovation. It has become increasingly clear that non-coding RNAs perform various regulatory functions in different cellular ... -
Amino acid identity at one position within the alpha1 helix of both the histidine kinase and the response regulator of the WalRK and PhoPR two-component systems plays a crucial role in the specificity of phosphotransfer.
(2010)Two-component systems usually function as cognate pairs, thereby ensuring an appropriate response to the detected signal. The ability to exclusively phosphorylate a partner protein, often in the presence of many competing ... -
Analysis of gene evolution and metabolic pathways using the Candida Gene Order Browser.
(BioMed Central, 2010)Background: Candida species are the most common cause of opportunistic fungal infection worldwide. Recent sequencing efforts have provided a wealth of Candida genomic data. We have developed the Candida Gene Order Browser ... -
Analysis of stage-specific gene perturbations and characterisation of two novel F-box genes during flower development in Arabidopsis thaliana
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Genetics, 2018)The model plant Arabidopsis thaliana has been used for past three decades to study the genetic and molecular processes underlying floral organogenesis. Flowers of this small plant consist of four concentric whorls, containing ... -
Analysis of the chloramphenicol resistance determinant of plasmid R26
(Trinity College (Dublin, Ireland). Department of Microbiology, 1985)The Inc P-1 plasmid, R2B carries an inducible chloramphenicol resistance determinant, cml. This specifies low level chloramphenicol resistance (30 μg/ml] in Escherichia coli. The mechanism does not involve drug detoxification ... -
Analysis of the function of the N1 subdomain of MSCRAMMs of Staphylococcus aureus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2014)The Microbial Surface Component Recognising Adhesive Matrix Molecules (MSCRAMMs) family of cell wall-associated proteins have recently been reclassified based on structure-function analysis. Under this new regime the MSCRAMM ... -
Ancient Goat Genomics: Structure, Selection, and Admixture
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Genetics, 2019)The wild bezoar Capra aegagrus was brought under human control c. 8,000 BC, leading to the domestic goat Capra hircus. This livestock species remains in use 10,000 years later, and is of particular importance to communities ... -
Angiogenin Levels and ANG Genotypes: Dysregulation in Amyotrophic Lateral Sclerosis
(2010)Objective: To determine whether 5 single nucleotide polymorphisms (SNPs) associate with ALS in 3 different populations. We also assessed the contribution of genotype to angiogenin levels in plasma and CSF. Methods: Allelic ... -
Anti-disease therapy for malaria: 'resistance proof'?
(2013)Antimalarial drugs have in the past fallen prey to resistance and this problem is likely to continue in the future. One approach to developing drugs that might be less prone to resistance might be to target the disease ...
