The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline.
Citation:
Coleman HA, Labrador JP, Chance RK, Bashaw GJ., The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline., Development., 137, 14, 2010, 2417-2426Download Item:
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Abstract:
Slits and their Roundabout (Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the
vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties. In a screen for genes involved in
Slit-Robo repulsion, we have identified the Adam family metalloprotease Kuzbanian (Kuz). Kuz does not regulate midline
repulsion through cleavage of Slit, nor is Slit cleavage essential for repulsion. Instead, Kuz acts in neurons to regulate repulsion
and Kuz can cleave the Robo extracellular domain in Drosophila cells. Genetic rescue experiments using an uncleavable form of
Robo show that this receptor does not maintain normal repellent activity. Finally, Kuz activity is required for Robo to recruit its
downstream signaling partner, Son of sevenless (Sos). These observations support the model that Kuz-directed cleavage is
important for Robo receptor activation
Sponsor
Grant Number
National Institutes of Health (NIH)
NS054739
National Institutes of Health (NIH)
NS046333
Wellcome Trust
Author's Homepage:
http://people.tcd.ie/labradojDescription:
PUBLISHEDPMID: 20570941
Author: Labrador, Juan
Type of material:
Journal ArticleCollections:
Series/Report no:
Development.137
14
Availability:
Full text availableKeywords:
Genetics, Axon guidance, DrosophilaDOI:
http://dx.doi.org/10.1242/dev.047993Licences: