H-NS silences gfp, the Green Fluorescent Protein gene: gfpTCD is a genetically remastered gfp gene with reduced susceptibility to H-NS-mediated transcription silencing and with enhanced translation
Citation:
Corcoran CP, Cameron ADS, Dorman CJ, H-NS silences gfp, the Green Fluorescent Protein gene: gfpTCD is a genetically remastered gfp gene with reduced susceptibility to H-NS-mediated transcription silencing and with enhanced translation, Journal of Bacteriology, 192, 18, 2010, 4790 - 4793Download Item:
0531-10.pdf (PDF) 1019.Kb
Abstract:
The bacterial nucleoid-associated protein H-NS, which preferentially targets and silences A+T-rich genes, binds the ubiquitous reporter gene gfp and dramatically reduces local transcription. We have redesigned gfp to reduce H-NS-mediated transcription silencing and simultaneously improve translation in vivo without altering the amino acid sequence of the GFP protein.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
Author's Homepage:
http://people.tcd.ie/cjdormanDescription:
PUBLISHEDPublished online July 16 2010
Author: DORMAN, CHARLES
Type of material:
Journal ArticleCollections:
Series/Report no:
Journal of Bacteriology192
18
Availability:
Full text availableKeywords:
MicrobiologySubject (TCD):
Genes & Society , Immunology, Inflammation & InfectionDOI:
http://dx.doi.org/10.1128/JB.00531-10Licences: