Layer-specific stem cell differentiation in tri-layered tissue engineering biomaterials: Towards development of a single-stage cell-based approach for osteochondral defect repair
Citation:
Levingstone, T.J. and Moran, C. and Almeida, H.V. and Kelly, D.J. and O'Brien, F.J., Layer-specific stem cell differentiation in tri-layered tissue engineering biomaterials: Towards development of a single-stage cell-based approach for osteochondral defect repair, Materials Today Bio, 12, 100173, 2021Download Item:
Abstract:
Successful repair of osteochondral defects is challenging, due in part to their complex gradient nature. Tissue
engineering approaches have shown promise with the development of layered scaffolds that aim to promote
cartilage and bone regeneration within the defect. The clinical potential of implanting these scaffolds cell-free has
been demonstrated, whereby cells from the host bone marrow MSCs infiltrate the scaffolds and promote cartilage
and bone regeneration within the required regions of the defect. However, seeding the cartilage layer of the
scaffold with a chondrogenic cell population prior to implantation may enhance cartilage tissue regeneration, thus
enabling the treatment of larger defects. Here the development of a cell seeding approach capable of enhancing
articular cartilage repair without the requirement for in vitro expansion of the cell population is explored. The
intrinsic ability of a tri-layered scaffold previously developed in our group to direct stem cell differentiation in
each layer of the scaffold was first demonstrated. Following this, the optimal chondrogenic cell seeding approach
capable of enhancing the regenerative capacity of the tri-layered scaffold was demonstrated with the highest
levels of chondrogenesis achieved with a co-culture of rapidly isolated infrapatellar fat pad MSCs (FPMSCs) and
chondrocytes (CCs). The addition of FPMSCs to a relatively small number of CCs led to a 7.8-fold increase in the
sGAG production over chondrocytes in mono-culture. This cell seeding approach has the potential to be delivered
within a single-stage approach, without the requirement for costly in vitro expansion of harvested cells, to achieve
rapid repair of osteochondral defects
Sponsor
Grant Number
Science Foundation Ireland (SFI)
TRA/2011/19
Author's Homepage:
http://people.tcd.ie/kellyd9
Author: Kelly, Daniel
Type of material:
Journal ArticleSeries/Report no:
Materials Today Bio12
100173
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Full text availableDOI:
http://dx.doi.org/10.1016/j.mtbio.2021.100173Metadata
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