STAT3 knockdown by siRNA induces apoptosis in human cutaneous T-cell lymphoma line Hut78 via downregulation of Bcl-xL
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2010Access:
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Verma, N.K., Davies, A.M., Long, A., Kelleher, D., Volkov, Y., STAT3 knockdown by siRNA induces apoptosis in human cutaneous T-cell lymphoma line Hut78 via downregulation of Bcl-xL, Cell Mol Biol Lett., 15, 2, 2010, 342 - 355Download Item:
Abstract:
Cutaneous T-cell lymphomas (CTCLs) are non-Hodgkin's
lymphomas resulting from clonal expansion and localization of malignant
T-lymphocytes to the skin. CTCL cells have defective apoptosis. Signal
transducers and activators of transcription (STAT) are a family of transcription
factors known to play important roles
in the development and progression of
several human cancers by promoting cell proliferation and protecting against
apoptosis. In this study, we investigated
the specific role of STAT3, a major
component of the STAT family, in growth and survival of human CTCL cell line
Hut78. Western immunoblot analysis showed elevated expression of STAT3 and
phospho-STAT3(Y705) in human CTCL cells as compared to freshly isolated
peripheral blood lymphocytes (PBLs). Specific knockdown of STAT3
expression in Hut78 cells by RNA interference induced morphological and
biochemical changes indicating apopt
otic cell death. Moreover, STAT3
inhibition downregulated the expression of
Bcl2 family of anti-apoptotic gene
Bcl-xL
. These observations suggest that STAT3
is required for the survival of
CTCL cells and strongly indicate that
targeting STAT3 using siRNA techniques
may serve a novel therapeutic strategy for the treatment of CTCL
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https://www.degruyter.com/view/j/cmble.2010.15.issue-2/s11658-010-0008-2/s11658-010-0008-2.xmlhttps://doi.org/10.2478/s11658-010-0008-2
http://hdl.handle.net/2262/90809
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http://people.tcd.ie/longaiDescription:
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Journal ArticleURI:
https://www.degruyter.com/view/j/cmble.2010.15.issue-2/s11658-010-0008-2/s11658-010-0008-2.xmlhttps://doi.org/10.2478/s11658-010-0008-2
http://hdl.handle.net/2262/90809
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Cell Mol Biol Lett.;15;
2;
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Full text availableKeywords:
Cutaneous T-cell lymphomas, Apoptosis, siRNA, STAT3Subject (TCD):
CancerLicences: