Riboflavin status modifies the effects of methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) polymorphisms on homocysteine
Citation:
García-Minguillán C.J, Fernandez-Ballart J.D, Ceruelo S, Ríos L, Bueno O, Berrocal-Zaragoza M.I, Molloy A.M, Ueland P.M, Meyer K, Murphy M.M, Riboflavin status modifies the effects of methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) polymorphisms on homocysteine, Genes and Nutrition, 9, 6, 2014, 435-Download Item:
12263_2014_Article_435.pdf (PDF) 483.4Kb
Abstract:
Methylenetetrahydrofolate reductase (MTHFR)
and methionine synthase red
uctase (MTRR), riboflavin-
dependent enzymes, participat
e in homocysteine metabolism.
Reported effects of riboflavin status on the association between
the
MTHFR
677C
[
T polymorphism and homocysteine vary,
and the effects of the
MTRR
66A
[
Gor
MTRR
524C
[
Tpoly-
morphisms on homocysteine are unclear. We tested the
hypothesis that the effects of the
MTHFR
677C
[
T,
MTRR
66A
[
Gand
MTRR
524C
[
T polymorphisms on fasting plasma
total homocysteine (tHcy) depen
d on riboflavin status (eryth-
rocyte glutathionine reductase
activation coefficient, optimum:
\
1.2; marginally deficient: 1.2–1.4; deficient:
C
1.4) in 771
adults aged 18–75 years.
MTHFR
677T allele carriers with
middle or low tertile plasma folate (
\
14.7 nmol/L) had 8.2 %
higher tHcy compared to the 677CC genotype (
p
\
0.01). This
effect was eliminated when riboflavin status was optimal (
p
for
interaction: 0.048). In the
lowest cobalamin quartile
(
B
273 pmol/L), riboflavin status modifies the relationship
between the
MTRR
66 A
[
G polymorphism and tHcy (
p
for
interaction: 0.034). tHcy was 6.6 % higher in
MTRR
66G allele
carriers compared to the 66AA
genotype with marginally
deficient or optimal riboflavin status, but there was no differ-
ence when riboflavin status was deficient (
p
for interaction:
0.059). tHcy was 13.7 % higher in
MTRR
524T allele carriers
compared to the 524CC genoty
pe when cobalamin status was
low (
p
\
0.01), but no difference was observed when we
stratified by riboflavin status. The effect of the
MTHFR
677C
[
T polymorphism on tHcy depends on riboflavin status,
that of the
MTRR
66A
[
G polymorphism on cobalamin and
riboflavin status and that of the
MTRR
524C
[
T polymorphism
on cobalamin status
Author's Homepage:
http://people.tcd.ie/amolloyDescription:
PUBLISHEDExport Date: 20 July 2015
Author: MOLLOY, ANNE
Type of material:
Journal ArticleCollections:
Series/Report no:
Genes and Nutrition9
6
Availability:
Full text availableDOI:
http://dx.doi.org/10.1007/s12263-014-0435-1Licences: