A Highly Unstable Transcript Makes CwlO D, L-Endopeptidase Expression Responsive to Growth Conditions in Bacillus subtilis
Citation:
Noone, D, Salzberg, LI, Botella, E, Basell, K, Becher, D, Antelmann, H, Devine, KM, A Highly Unstable Transcript Makes CwlO D, L-Endopeptidase Expression Responsive to Growth Conditions in Bacillus subtilis, JOURNAL OF BACTERIOLOGY, 196, 2, 2014, 237-247Download Item:
Abstract:
The Bacillus subtilis cell wall is a dynamic structure, composed of peptidoglycan and teichoic acid, that is continually remodeled during growth. Remodeling is effected by the combined activities of penicillin binding proteins and autolysins that participate in the synthesis and turnover of peptidoglycan, respectively. It has been established that one or the other of the CwlO and LytE D,L-endopeptidase-type autolysins is essential for cell viability, a requirement that is fulfilled by coordinate control of their expression by WalRK and SigI RsgI. Here we report on the regulation of cwlO expression. The cwlO transcript is very unstable, with its degradation initiated by RNase Y cleavage within the 187-nucleotide leader sequence. An antisense cwlO transcript of heterogeneous length is expressed from a SigB promoter that has the potential to control cellular levels of cwlO RNA and protein under stress conditions. We discuss how a multiplicity of regulatory mechanisms makes CwlO expression and activity responsive to the prevailing growth conditions.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
08/1N.1/B185
Author's Homepage:
http://people.tcd.ie/kdevineDescription:
PUBLISHED
Author: DEVINE, KEVIN
Type of material:
Journal ArticleCollections
Series/Report no:
JOURNAL OF BACTERIOLOGY196
2
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Full text availableKeywords:
Bacillus subtilis.DOI:
http://dx.doi.org/10.1128/JB.00986-13ISSN:
0021-9193Metadata
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