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dc.contributor.authorDEVINE, KEVINen
dc.date.accessioned2014-10-20T13:28:55Z
dc.date.available2014-10-20T13:28:55Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationNoone, D, Salzberg, LI, Botella, E, Basell, K, Becher, D, Antelmann, H, Devine, KM, A Highly Unstable Transcript Makes CwlO D, L-Endopeptidase Expression Responsive to Growth Conditions in Bacillus subtilis, JOURNAL OF BACTERIOLOGY, 196, 2, 2014, 237-247en
dc.identifier.issn0021-9193en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/71608
dc.descriptionPUBLISHEDen
dc.description.abstractThe Bacillus subtilis cell wall is a dynamic structure, composed of peptidoglycan and teichoic acid, that is continually remodeled during growth. Remodeling is effected by the combined activities of penicillin binding proteins and autolysins that participate in the synthesis and turnover of peptidoglycan, respectively. It has been established that one or the other of the CwlO and LytE D,L-endopeptidase-type autolysins is essential for cell viability, a requirement that is fulfilled by coordinate control of their expression by WalRK and SigI RsgI. Here we report on the regulation of cwlO expression. The cwlO transcript is very unstable, with its degradation initiated by RNase Y cleavage within the 187-nucleotide leader sequence. An antisense cwlO transcript of heterogeneous length is expressed from a SigB promoter that has the potential to control cellular levels of cwlO RNA and protein under stress conditions. We discuss how a multiplicity of regulatory mechanisms makes CwlO expression and activity responsive to the prevailing growth conditions.en
dc.format.extent237-247en
dc.language.isoenen
dc.relation.ispartofseriesJOURNAL OF BACTERIOLOGYen
dc.relation.ispartofseries196en
dc.relation.ispartofseries2en
dc.rightsYen
dc.subjectBacillus subtilis.en
dc.titleA Highly Unstable Transcript Makes CwlO D, L-Endopeptidase Expression Responsive to Growth Conditions in Bacillus subtilisen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kdevineen
dc.identifier.rssinternalid96054en
dc.identifier.doihttp://dx.doi.org/10.1128/JB.00986-13en
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorGrantNumber08/1N.1/B185en


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