dc.contributor.author | Labrador, Juan | en |
dc.date.accessioned | 2011-06-01T15:29:41Z | |
dc.date.available | 2011-06-01T15:29:41Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Coleman HA, Labrador JP, Chance RK, Bashaw GJ., The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline., Development., 137, 14, 2010, 2417-2426 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/56260 | |
dc.description | PUBLISHED | en |
dc.description | PMID: 20570941 | en |
dc.description.abstract | Slits and their Roundabout (Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the
vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties. In a screen for genes involved in
Slit-Robo repulsion, we have identified the Adam family metalloprotease Kuzbanian (Kuz). Kuz does not regulate midline
repulsion through cleavage of Slit, nor is Slit cleavage essential for repulsion. Instead, Kuz acts in neurons to regulate repulsion
and Kuz can cleave the Robo extracellular domain in Drosophila cells. Genetic rescue experiments using an uncleavable form of
Robo show that this receptor does not maintain normal repellent activity. Finally, Kuz activity is required for Robo to recruit its
downstream signaling partner, Son of sevenless (Sos). These observations support the model that Kuz-directed cleavage is
important for Robo receptor activation | en |
dc.description.sponsorship | We thank Kim Bland and Corey Goodman for generating the uncleavable Slit
construct, John Flanagan for the mouse ADAM10 reagents, D. J. Pan for the
Kuzbanian dominant-negative transgenes and the Bloomington Stock Center
for Drosophila strains. We are grateful to members of the Bashaw lab for
thoughtful discussion and to Andy McClelland for assistance with the
quantification of the Sos recruitment assay. This work was supported by a
Whitehall Foundation Research Grant, a Burroughs Wellcome Career Award,
and NIH grants NS046333 and NS054739 to G.J.B. Deposited in PMC for
release after 12 months. | en |
dc.format.extent | 2417-2426 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Development. | en |
dc.relation.ispartofseries | 137 | en |
dc.relation.ispartofseries | 14 | en |
dc.relation.uri | https://scholar.google.com/citations?view_op=view_citation&hl=en&user=nUwp_fcAAAAJ&citation_for_view=nUwp_fcAAAAJ:Tyk-4Ss8FVUC | en |
dc.rights | Y | en |
dc.subject | Genetics | en |
dc.subject | Axon guidance | en |
dc.subject | Drosophila | en |
dc.title | The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline. | en |
dc.type | Journal Article | en |
dc.contributor.sponsor | National Institutes of Health (NIH) | en |
dc.contributor.sponsor | National Institutes of Health (NIH) | en |
dc.contributor.sponsor | Wellcome Trust | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/labradoj | en |
dc.identifier.rssinternalid | 71158 | en |
dc.identifier.doi | http://dx.doi.org/10.1242/dev.047993 | en |
dc.contributor.sponsorGrantNumber | NS054739 | en |
dc.contributor.sponsorGrantNumber | NS046333 | en |
dc.relation.cites | Cites | en |
dc.identifier.rssuri | http://dev.biologists.org/content/137/14/2417.long | en |