Migration of lymphoma and lymphocyte T cells on flat and grooved microfabricated surfaces : comparative analysis of peripheral blood T lymphocytes and lymphoma human T cell line HUT78
Citation:
Adriele Prino-Mello, 'Migration of lymphoma and lymphocyte T cells on flat and grooved microfabricated surfaces : comparative analysis of peripheral blood T lymphocytes and lymphoma human T cell line HUT78', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Medicine, 2003, pp 477Download Item:
PrinoMello TCD THESIS 7349 Migration of.pdf (PDF) 257.9Mb
Abstract:
Normal and malignant cell migration represents a fundamentally important process involved in tissue growth and development, inflammation and cancer metastasis. Leukocyte trafficking from blood into tissues is currently described as a multi-step process that involves initial tethering and rolling, firm adhesion, polarisation and subsequently active locomotion through the vascular endothelium. A specialized leukocyte population comprising T lymphocytes and involved in cell-mediated immunity has a major impact on the overall efficiency of host defence. A number of factors have been previously reported to contribute to the success of directed T cell migration towards effector sites in tissues and organs. These physiological variables include expression of specific cell surface receptor repertoire, chemoattractants and extracellular matrix composition providing both natural migration barriers and tissue-specific microenvironment architecture. In this thesis we have tested the hypothesis that migration of different T cell types could be influenced by specific topographical geometry of the substrate as one of the contributing microenvironmental factors. Therefore, the objective of this study was to quantify the extent of difference in the locomotory response of malignant cells (HUT78 T lymphoma cell line) and normal peripheral blood T lymphocyte cells (PBTL) to microfabricated surfaces with distinctive custom-produced topographical patterns.
Author: Prino-Mello, Adriele
Advisor:
Prendegast, PatrickQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical MedicineNote:
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