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dc.contributor.authorDORMAN, CHARLES
dc.date.accessioned2016-10-04T14:02:57Z
dc.date.available2016-10-04T14:02:57Z
dc.date.issued2015en
dc.date.submitted2015en
dc.identifier.citationCHARLES DORMAN, 'Re-engineering cellular physiology by rewiring high-level global regulatory genes', 2015, Scientific Reports;, 5;en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/77462
dc.description.abstractKnowledge of global regulatory networks has been exploited to rewire the gene control programmes of the model bacterium Salmonella enterica serovar Typhimurium. The product is an organism with competitive fitness that is superior to that of the wild type but tuneable under specific growth conditions. The paralogous hns and stpA global regulatory genes are located in distinct regions of the chromosome and control hundreds of target genes, many of which contribute to stress resistance. The locations of the hns and stpA open reading frames were exchanged reciprocally, each acquiring the transcription control signals of the other. The new strain had none of the compensatory mutations normally associated with alterations to hns expression in Salmonella; instead it displayed rescheduled expression of the stress and stationary phase sigma factor RpoS and its regulon. Thus the expression patterns of global regulators can be adjusted artificially to manipulate microbial physiology, creating a new and resilient organism.en
dc.description.sponsorshipThis work was supported by Science Foundation Ireland Principal Investigator Award 13/IA/1875 to CJD and the Saskatchewan Health Research Foundation [Grant 2867] and Natural Sciences and Engineering Research Council of Canada [Discovery Grant 435784-2013] to ADSC.en
dc.format.extent17653en
dc.language.isoenen
dc.relation.ispartofseriesScientific Reports;
dc.relation.ispartofseries5;
dc.rightsYen
dc.subjectbacterium Salmonella enterica serovar Typhimuriumen
dc.subject.lcshbacterium Salmonella enterica serovar Typhimuriumen
dc.titleRe-engineering cellular physiology by rewiring high-level global regulatory genesen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/cjdorman
dc.identifier.rssinternalid107178
dc.identifier.doihttp://dx.doi.org/10.1038/srep17653
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorGrantNumber13 IA 1875en
dc.subject.TCDThemeGenes & Societyen
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.identifier.orcid_id0000-0002-6018-9170


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