Human Vδ2+ γδ T cells differentially induce maturation, cytokine production, and alloreactive T cell stimulation by dendritic cells and B cells
File Type:
PDFItem Type:
Journal ArticleDate:
2014Access:
openAccessCitation:
Petrasca A, Doherty DG, Human Vδ2+ γδ T cells differentially induce maturation, cytokine production, and alloreactive T cell stimulation by dendritic cells and B cells, Frontiers in Immunology, 2014, 650-Download Item:
Abstract:
Human γδ T cells expressing the Vγ9Vδ2 T cell receptor can induce maturation of dendritic cells (DC) into antigen-presenting cells (APC) and B cells into antibody-secreting plasma cells. Since B cells are capable of presenting antigens to T cells, we investigated if Vγ9Vδ2 T cells can influence antigen-presentation by these cells. We report that Vγ9Vδ2 T cells induced expression of CD86, HLA-DR, and CD40 by B cells and stimulated the release of IL-4, IL-6, TNF-α, and IgG, IgA, and IgM. Vγ9Vδ2 T cells also augmented the ability of B cells to stimulate proliferation but not IFN-γ or IL-4 release by alloreactive T cells. In contrast, Vγ9Vδ2 T cells induced expression of CD86 and HLA-DR and the release of IFN-γ, IL-6, and TNF-α by DC and these DC stimulated proliferation and IFN-γ production by conventional T cells. Furthermore, CD86, TNF-α, IFN-γ, and cell contact were found to be important in DC activation by Vγ9Vδ2 T cells but not in the activation of B cells. These data suggest that Vγ9Vδ2 T cells can induce maturation of B cells and DC into APC, but while they prime DC to stimulate T helper 1 (TH1) responses, they drive maturation of B cells into APC that can stimulate different T cell responses. Thus, Vγ9Vδ2 T cells can control different arms of the immune system through selective activation of B cells and DC in vitro, which may have important applications in immunotherapy and for vaccine adjuvants.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
Author's Homepage:
http://people.tcd.ie/dohertdehttp://people.tcd.ie/petrasan
Description:
PUBLISHED
Author: Doherty, Derek; Phelan, Andreea
Type of material:
Journal ArticleCollections
Series/Report no:
Frontiers in ImmunologyAvailability:
Full text availableKeywords:
T cell proliferation, APC, antibody production,, cytokines,, B cells,, dendritic cells,, human γδ T cellsSubject (TCD):
Immunology, Inflammation & Infection , ANTIBODIES , ANTIGEN-PRESENTING CELLS , CYTOKINE SECRETION , CYTOKINES , HUMAN DENDRITIC CELLS , IMMUNOGLOBULIN , T-CELL ACTIVATION , T-CELL PROLIFERATION , gamma delta T cellsDOI:
http://dx.doi.org/10.3389/fimmu.2014.00650Metadata
Show full item recordLicences: