A transcription factor network coordinates attraction, repulsion, and adhesion combinatorially to control motor axon pathway selection.
Item Type:Journal Article
Citation:Zarin AA, Asadzadeh J, Hokamp K, McCartney D, Yang L, Bashaw GJ, Labrador JP, A transcription factor network coordinates attraction, repulsion, and adhesion combinatorially to control motor axon pathway selection., Neuron, 81, 6, 2014, 1297-311
nihms-615700.pdf (Accepted for publication (author's copy) - Peer Reviewed) 2.011Mb
Combinations of transcription factors (TFs) instruct precise wiring patterns in the developing nervous system; however, how these factors impinge on surface molecules that control guidance decisions is poorly understood. Using mRNA profiling, we identified the complement of membrane molecules regulated by the homeobox TF Even-skipped (Eve), the major determinant of dorsal motor neuron (dMN) identity in Drosophila. Combinatorial loss- and gain-of-function genetic analyses of Eve target genes indicate that the integrated actions of attractive, repulsive, and adhesive molecules direct eve-dependent dMN axon guidance. Furthermore, combined misexpression of Eve target genes is sufficient to partially restore CNS exit and can convert the guidance behavior of interneurons to that of dMNs. Finally, we show that a network of TFs, comprised of eve, zfh1, and grain, induces the expression of the Unc5 and Beaten-path guidance receptors and the Fasciclin 2 and Neuroglian adhesion molecules to guide individual dMN axons.
Science Foundation Ireland (SFI)
Type of material:Journal Article
Availability:Full text available
Keywords:f transcription factors (TFs)