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dc.contributor.authorFARES, MARIOen
dc.date.accessioned2013-09-02T13:32:52Z
dc.date.available2013-09-02T13:32:52Z
dc.date.issued2009en
dc.date.submitted2009en
dc.identifier.citationToft, C., Williams, T.A., Fares, M.A, Genome-Wide Functional Divergence after the Symbiosis of Proteobacteria with Insects Unraveled through a Novel Computational Approach, PLoS Computational Biology, 5, 4, 2009, e1000344en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/67303
dc.descriptionPUBLISHEDen
dc.descriptionPubMed ID: 19343224en
dc.description.abstractSymbiosis has been among the most important evolutionary steps to generate biological complexity. The establishment of symbiosis required an intimate metabolic link between biological systems with different complexity levels. The strict endo- cellular symbiotic bacteria of insects are beautiful examples of the metabolic coupling between organisms belonging to different kingdoms, a eukaryote and a prokaryote. The host (eukaryote) provides the endosymbiont (prokaryote) with a stable cellular environment while the endosymbiont supplements the host's diet with essential metabolites. For such communication to take place, endosymbionts' genomes have suffered dramatic modifications and reconfigurations of proteins' functions. Two of the main modifications, loss of genes redundant for endosymbiotic bacteria or the host and bacterial genome streamlining, have been extensively studied. However, no studies have accounted for possible functional shifts in the endosymbiotic proteomes. Here, we develop a simple method to screen genomes for evidence of functional divergence between two species clusters, and we apply it to identify functional shifts in the endosymbiotic proteomes. Despite the strong effects of genetic drift in the endosymbiotic systems, we unexpectedly identified genes to be under stronger selective constraints in endosymbionts of aphids and ants than in their free-living bacterial relatives. These genes are directly involved in supplementing the host's diet with essential metabolites. A test of functional divergence supports a strong relationship between the endosymbiosis and the functional shifts of proteins involved in the metabolic communication with the insect host. The correlation between functional divergence in the endosymbiotic bacterium and the ecological requirements of the host uncovers their intimate biochemical and metabolic communication and provides insights on the role of symbiosis in generating species diversity.en
dc.description.sponsorshipThis work was supported by Science Foundation Ireland Grant to MAF and a grant from the Irish Council for Science Engineering and Technology to CT and TAW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscripten
dc.format.extente1000344en
dc.language.isoenen
dc.relation.ispartofseriesPLoS Computational Biologyen
dc.relation.ispartofseries5en
dc.relation.ispartofseries4en
dc.rightsYen
dc.subjectsymbiosisen
dc.subject.lcshsymbiosisen
dc.titleGenome-Wide Functional Divergence after the Symbiosis of Proteobacteria with Insects Unraveled through a Novel Computational Approachen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/faresmen
dc.identifier.rssinternalid87383en
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pcbi.1000344en


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