The neural cell adhesion molecule-derived peptide, FGL, attenuates lipopolysaccharide-induced changes in glia in a CD200-dependent manner.
Citation:
Cox FF, Berezin V, Bock E, Lynch MA, The neural cell adhesion molecule-derived peptide, FGL, attenuates lipopolysaccharide-induced changes in glia in a CD200-dependent manner., Neuroscience, 235, 2013, 141-148Download Item:
Abstract:
Fibroblast growth loop (FGL) is a neural cell adhesion molecule (NCAM)-mimetic peptide
that mimics the interaction of NCAM with fibroblast growth factor receptor (FGFR). FGL
increases neurite outgrowth and promotes neuronal survival in vitro, and it has also been
shown to have neuroprotective effects in vivo. More recent evidence has indicated that FGL
has anti-inflammatory effects, decreasing age-related changes in microglial activation and
production of inflammatory cytokines. These changes have been associated with an FGLinduced
increase in expression of the glycoprotein, CD200, which interacts with its receptor
to help maintain microglia in a quiescent state. However whether the FGL-induced antiinflammatory
effects are CD200-dependent has not been examined. The objective of this
study was to address this question. Mixed glia were prepared from brain tissue of neonatal
wildtype and CD200-deficient mice and preincubated with FGL prior to stimulation with
lipopolysaccharide (LPS). Cells were assessed for mRNA expression of markers of
microglial activation, CD11b, CD40 and intercellular adhesion molecule 1 (ICAM) and also
the inflammatory cytokines, interleukin (IL)-1?, IL-6 and tumour necrosis factor (TNF)-?,
while supernatant concentrations of these cytokine were also assessed. LPS significantly
increased all these parameters and the effect was greater in cells prepared from CD200-
deficient mice. Whereas FGL attenuated the LPS-induced changes in cells from wildtype
mice, it did not do so in cells from CD200-deficient mice. We conclude that the FGL-induced
changes in microglial activation are CD200-dependent and demonstrate that the interaction of
astrocytes with microglia is critically important for modulating microglial activation
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http://people.tcd.ie/lynchmaDescription:
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Author: LYNCH, MARINA ANNETTA
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Neuroscience;235;
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