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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/64051

Title: Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis.
Author: FLEMING, ALASTAIR
Author's Homepage: http://people.tcd.ie/fleminal
Keywords: incubation
Extracellular Enzyme Synthesis
Bacillus licheniformis
enzymes
RNA
Issue Date: 1995
Publisher: American Society for Microbiology
Citation: Fleming AB, Tangney M, Jørgensen PL, Diderichsen B, Priest FG., Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis., Applied and environmental microbiology, 61, 11, 1995, 3775 - 3780
Series/Report no.: Applied and environmental microbiology;61, 11
Abstract: A deletion of the spoIIAC gene of Bacillus licheniformis was prepared in vitro by using the splicing-by-overlapextension technique. This gene was introduced into B. licheniformis on a temperature-sensitive plasmid, and following integration and excision from the chromosome, a precisely located deletion on the chromosomal gene was prepared. The mutated bacterium was totally asporogenous and formed abortively disporic cells characterized by asymmetric septa at the poles of the cells. Qualitative plate tests revealed that the bacterium synthesized normal levels of DNase, polygalacturonate lyase, protease, RNase, and xylanase, but the hydrolysis zones due to b-1,3-glucanase and carboxymethyl cellulase activity were smaller in the mutant than in the parent strain. The synthesis of alkaline protease was the same in batch cultures of the mutant and the parent during prolonged incubation for 72 h, but the a-amylase yields were reduced by about 30% by the mutation.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/64051
Related links: http://aem.asm.org/cgi/reprint/61/11/3775
Appears in Collections:Microbiology (Scholarly Publications)

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