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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/62432

Title: The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid
Author: GEOGHEGAN, JOAN
FOSTER, TIMOTHY JAMES
Sponsor: 
Name Grant Number
WT084483
RP/2007/3
08/IN.1/B1854

Author's Homepage: http://people.tcd.ie/tfoster
http://people.tcd.ie/geoghejo
Keywords: Immunology
Staphylococcus aureus
Issue Date: 2012
Publisher: John Wiley
Citation: Smith EJ, Corrigan RM, van der Sluis T, Gründling A, Speziale P, Geoghegan JA, Foster TJ, The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid, Molecular Microbiology, 83, 4, 2012, 789 - 804
Series/Report no.: Molecular Microbiology;
83;
4;
Abstract: The Sbi protein of Staphylococcus aureus comprises two IgG binding domains similar to those of protein A and a region that triggers the activation of complement C3. Sbi is expressed on the cell surface but its C-terminal domain lacks motifs associated with wall or membrane anchoring of proteins in Gram-positive bacteria. Cell-associated Sbi fractionates with the cytoplasmic membrane and is not solubilised during protoplast formation. S.aureus expressing Sbi truncates of the C-terminal Y domain allowed identification of residues that are required for association of Sbi with the membrane. Recombinant Sbi bound to purified cytoplasmic membrane material in vitro and to purified lipoteichoic acid. This explains how Sbi partitions with the membrane in fractionation experiments yet is partially exposed on the cell surface. An LTA-defective mutant of S. aureus had reduced levels of Sbi in the cytoplasmic membrane.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/62432
Related links: http://dx.doi.org/10.1111/j.1365-2958.2012.07966.x
Appears in Collections:Microbiology (Scholarly Publications)

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