Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics.
Citation:Gerard VA, Maguire CM, Bazou D, Gun'ko YK, Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics., Journal of Nanobiotechnology, 9, 1, 2011, 50
Folic acid modified gelatine coated quantum dots as.pdf (Published (publisher's copy) - Peer Reviewed) 1.851Mb
BACKGROUND: Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). RESULTS: The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. CONCLUSIONS: The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics.
Higher Education Authority (HEA)
Science Foundation Ireland (SFI)
Type of material:Journal Article
Series/Report no:Journal of Nanobiotechnology
Availability:Full text available