Substituted dipyridophenazine complexes of Cr(III): synthesis, enantiomeric resolution and binding interactions with calf thymus DNA.
Citation:
Vasudevan S, Smith JA, Wojdyla M, DiTrapani A, Kruger PE, McCabe T, Fletcher NC, Quinn SJ, Kelly JM, Substituted dipyridophenazine complexes of Cr(III): synthesis, enantiomeric resolution and binding interactions with calf thymus DNA., Dalton Transactions, 39, 16, 2010, 3990-8Download Item:
Substituted dipyridophenazine complexes of Cr(III)- Synthesis, enantiomeric resolution and binding interactions with calf thymus DNA.pdf (Published (publisher's copy) - Peer Reviewed) 599.3Kb
Abstract:
[Cr(phen)2(X2dppz)]3+ {X = H, Me, or F} have been synthesised, characterised, and chromatographically resolved into their constituent ? and ? enantiomers. The DNA-binding interactions of each of the racemic complexes were investigated, with the results of linear dichroism, thermal denaturation, and emission quenching studies indicative of intercalative binding to CT-DNA with a significant electrostatic contribution. UV/Vis absorption titrations suggest strong DNA binding by each of the racemic complexes, with the methylated analogue [Cr(phen)2(Me2dppz)]3+ exhibiting the largest equilibrium binding constant. Emission quenching and UV-Vis titrations of the enantiomers of [Cr(phen)2(dppz)]3+ imply similar binding affinities for the ? and ? isomers, although significant differences between the circular dichroism spectra of the enantiomers in the presence of DNA connote differences in binding orientation and/or conformation between the two.
Sponsor
Grant Number
Higher Education Authority (HEA)
PRLTI4/CSCB
Science Foundation Ireland (SFI)
07/CHEF437
Science Foundation Ireland (SFI)
06/CHP036
Irish Research Council for Science and Engineering Technology (IRCSET)
Author's Homepage:
http://people.tcd.ie/tmccabehttp://people.tcd.ie/jmkelly
Description:
PUBLISHED
Author: KELLY, JOHN; MCCABE, THOMAS
Type of material:
Journal ArticleCollections:
Series/Report no:
Dalton Transactions39
16
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Full text availableSubject (TCD):
Genes & SocietyDOI:
http://dx.doi.org/10.1039/c000150cISSN:
1477-9226Licences: