Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
Citation:Creagh EM, Brumatti G, Sheridan C, Duriez PJ, Taylor RC, Cullen SP, Adrain C, Martin SJ, Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival., PloS one, 4, 3, 2009, e5055
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Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/?NAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, ?NAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/?NAC is essential for cell survival and is a conserved target of caspases from flies to man
Type of material:Journal Article
Series/Report no:PloS one
Availability:Full text available