Compensatory evolution of gene regulation in response to stress by Escherichia coli lacking RpoS.
Citation:Stoebel DM, Hokamp K, Last MS, Dorman CJ, Compensatory evolution of gene regulation in response to stress by Escherichia coli lacking RpoS., PLoS Genetics, 5, 10, 2009, e1000671
Compensatory Evolution of Gene Regulation in Response to Stress by Escherichia coli Lacking RpoS.pdf (Published (publisher's copy) - Peer Reviewed) 258.1Kb
Compensatory evolution of gene regulation in response to stress by Escherichia coli lacking RpoS.xml (Published (publisher's copy) - Peer Reviewed) 104.4Kb
The RpoS sigma factor protein of Escherichia coli RNA polymerase is the master transcriptional regulator of physiological responses to a variety of stresses. This stress response comes at the expense of scavenging for scarce resources, causing a trade-off between stress tolerance and nutrient acquisition. This trade-off favors non-functional rpoS alleles in nutrient-poor environments. We used experimental evolution to explore how natural selection modifies the regulatory network of strains lacking RpoS when they evolve in an osmotically stressful environment. We found that strains lacking RpoS adapt less variably, in terms of both fitness increase and changes in patterns of transcription, than strains with functional RpoS. This phenotypic uniformity was caused by the same adaptive mutation in every independent population: the insertion of IS10 into the promoter of the otsBA operon. OtsA and OtsB are required to synthesize the osmoprotectant trehalose, and transcription of otsBA requires RpoS in the wild-type genetic background. The evolved IS10 insertion rewires expression of otsBA from RpoS-dependent to RpoS-independent, allowing for partial restoration of wild-type response to osmotic stress. Our results show that the regulatory networks of bacteria can evolve new structures in ways that are both rapid and repeatable.
Type of material:Journal Article
Series/Report no:PLoS Genetics
Availability:Full text available