Transplantation of syngeneic schwann cells to the retina of the rhodopsin knockout (Rho-/-) mouse
Item Type:Journal Article
Citation:Keegan D, Kenna PF, Humphries MM, Humphries P, Flitcroft DI, Coffey PJ, Lund RD and Lawrence JM, `Transplantation of syngeneic schwann cells to the retina of the rhodopsin knockout (Rho-/-) mouse? in Investigative Ophthalmology and Visual Science, 44, (8), 2003, pp 3526 - 3532
10.Transplantation of syngeneic Schwann cells to the retina of the rhodopsin knockout (rho(---)) mouse.pdf (published (publisher copy) peer-reviewed) 436.7Kb
PURPOSE: To determine whether subretinal Schwann cell transplantation can prolong the survival of photoreceptors in the rhodopsin knockout (rho(-/-)) mouse. METHODS: Schwann cells were prepared from postnatal day (PN) 5 to 7 mouse pups and grafted subretinally into the eyes of PN35 rho(-/-) mice. RT-PCR was performed on similarly prepared cells to determine growth factor production in vitro. Eyes were retrieved at PN70 for anatomic and statistical analysis. Control animals received grafts of fibroblasts or sham surgery. RESULTS: RT-PCR demonstrated the presence of message for ciliary neurotrophic factor (CNTF), brain-derived neurotrophic factor (BDNF), and glia-derived neurotrophic factor (GDNF) in the cultured Schwann cells. Schwann cell grafts produced a statistically significant rescue of photoreceptors in a restricted area of retina at PN70, but the effect was lost by PN140. Preserved inner segments could be identified, but outer segments were never present. Sham surgery also resulted in photoreceptor rescue but at a reduced level. Fibroblast grafts appeared to produce little or no rescue effect. Grafts of Schwann cells or fibroblasts and sham surgery induced a reactive Muller glial response. CONCLUSIONS: Schwann cells can prolong photoreceptor survival in the rhodopsin knockout mouse until at least PN70.
Health Research Board
Medical Research Council
Author: HUMPHRIES, PETER
Publisher:The Association for Research in Vision and Ophthalmology, Inc.
Type of material:Journal Article
Series/Report no:Investigative Ophthalmology and Visual Science
Availability:Full text available