Development of liquid culture media mimicking the conditions of sinuses and lungs in cystic fibrosis and health
Citation:
Siobhan O'Brien, Development of liquid culture media mimicking the conditions of sinuses and lungs in cystic fibrosis and health, F1000Research, 11, 2022, 1007Download Item:
Abstract:
The respiratory tract is a compartmentalised and heterogenous
environment. The nasopharynx and sinuses of the upper airways have
distinct properties from the lungs and these differences may shape
bacterial adaptation and evolution. Upper airway niches act as early
colonisation sites for respiratory bacterial pathogens, including those,
such as Pseudomonas aeruginosa, that can go on to establish chronic
infection of the lungs in people with cystic fibrosis (CF). Despite the
importance of upper airway environments in facilitating early
adaptation to host environments, currently available in vitro models
for study of respiratory infection in CF focus exclusively on the lungs.
Furthermore, animal models, widely used to bridge the gap between
in vitro systems and the clinical scenario, do not allow the upper and
lower airways to be studied in isolation. We have developed a suite of
culture media reproducing key features of the upper and lower
airways, for the study of bacterial adaptation and evolution in
different respiratory environments. For both upper and lower airwaymimicking media, we have developed formulations that reflect airway
conditions in health and those that reflect the altered environment of
the CF respiratory tract. Here, we describe the development and
validation of these media and their use for study of genetic and
phenotypic adaptations in P. aeruginosa during growth under upper or
lower airway conditions in health and in CF.
Author's Homepage:
http://people.tcd.ie/obries79Description:
PUBLISHED
Author: O'Brien, Siobhan
Type of material:
Journal ArticleCollections
Series/Report no:
F1000Research;11;
Availability:
Full text availableKeywords:
Cystic Fibrosis (CF), sinuses, lungs, in vitro models, 3RsSubject (TCD):
Immunology, Inflammation & Infection , MICROBIOLOGY , Microbial ecologyDOI:
http://dx.doi.org/10.12688/f1000research.125074.1Metadata
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