Investigating the Impact of the Non-Structural Proteins of Respiratory Syncytial Virus on the Type I Interferon Response
Citation:
Efstathiou, Claudia Mary, Investigating the Impact of the Non-Structural Proteins of Respiratory Syncytial Virus on the Type I Interferon Response , Trinity College Dublin.School of Biochemistry & Immunology, 2023Download Item:
C_Efstathiou_thesis_0323.pdf (Published (publisher's copy)) 10.19Mb
Abstract:
Respiratory syncytial virus is the leading cause of bronchiolitis in infants, causing 33.2
million hospital admissions and over 118,000 deaths each year. The primary site of
RSV infection are the epithelial cells of the respiratory tract. On infection RSV evades
the immune response through several mechanisms, including subversion of responses
to the anti-viral cytokine IFNα by interacting with the JAK-STAT pathway. IFNα is a
potent antiviral cytokine that quickly causes an up-regulation in over 500 IFN stimulated
genes to make the cell less permissive to infection and viral growth. The action of RSV
prevents the up-regulation of ISGs, including MxA, ISG15, USP18 and PKR, which
prevents infected cells from mounting a robust anti-viral response. Our research focuses
on the immune evasive role of RSV?s two non-structural proteins, NS1 and NS2, and
the mechanism by which they limit JAK-STAT signalling.
We expressed NS1 and NS2 in human alveolar epithelial cells (A549) and bronchial
epithelial cells (BEAS 2b) and analysed their effect upon the JAK-STAT pathway. We
discovered that expressing NS1 in A549 and BEAS 2b cells lead to a significant reduction
in ISGs, while NS2 had no significant effect. There was no matched reduction in pSTAT1
or pSTAT2 in either cell line. However, BEAS 2b had increased STAT1 and STAT2
phosphorylation with NS1 expression. By studying the subcellular localization of STAT1
we have shown that expression of NS1 reduced STAT1 trafficking to the nucleus, and
altered STAT2 localization, providing a new mechanism by which RSV NS1 prevents
efficient JAK-STAT signalling. While the primary site of RSV infection is epithelial cells
of the upper respiratory track, there is some evidence that immune cells can be infected
by RSV. To examine the extent of the permissiveness of circulating immune cells, adult
PBMCs were incubated with RSV and the resulting infection measured. We found that
around 2% of lymphocytes became infected and that there was a reduction in IFNα
sensitivity after RSV infection. This reduction in responsiveness to IFNα may result in
delayed viral clearance and altered phenotypic differentiation.
Taken together, this work has shown that RSV NS proteins have key immune evasion
roles that are used to overcome the IFNα response by reducing the signalling through
the JAK-STAT pathway. The ability of RSV NS proteins to limit the activity of IFNα
identifies the NS proteins as targets for therapeutic treatments for RSV. Our work gives
a better understanding of how RSV NS proteins acts to subvert the immune response in
the most clinically relevant cell types.
Sponsor
Grant Number
National Children's Hospital
Higher Education Authority (HEA)
Author's Homepage:
https://tcdlocalportal.tcd.ie/pls/EnterApex/f?p=800:71:0::::P71_USERNAME:EFSTATHCDescription:
APPROVED
Author: Efstathiou, Claudia Mary
Advisor:
Stevenson, NigelPublisher:
Trinity College Dublin. School of Biochemistry & Immunology. Discipline of BiochemistryType of material:
ThesisAvailability:
Full text availableKeywords:
Respiratory Syncytial Virus, JAK/STAT, Signalling, Virology, ImmunologyLicences: