Deletions in the r-determinant mer region of plasmid R100-1 selected by loss of mercury hypersensitivity
Citation:
Foster, T.J. and Nakahara, H., Deletions in the r-determinant mer region of plasmid R100-1 selected by loss of mercury hypersensitivity, Journal of Bacteriology, 1979, 140, 1, 301 - 305Download Item:
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Abstract:
A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to be selected which resulted in either reversion to Hg2+ resistance (characteristic plasmid R100-1) or sensitivity at a level characteristic of plasmidless strains. Restriction endonuclease EcoRI and BamHI analysis showed that reversion to resistance resulted from loss of TnA from the R100-mer:Tn801 plasmid, whereas the change from hypersensitivity to sensitivity to Hg2+ usually resulted from deletion of part or all of Tn801 plus plasmid deoxyribonucleic acid sequences corresponding to the operator-proximal end of the mer operon.
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Author: FOSTER, TIMOTHY
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Journal of Bacteriology;140;
1;
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