Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice
Citation:
Orlagh M. Kelly, Richard K. Porter, Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1807, 9, 2011, 1064-1074Download Item:
Absence of mitochondrial uncoupling protein 3- Effect on thymus and spleen in the fed and fasted mice.pdf (Published (author's copy) - Peer Reviewed) 836.1Kb
Abstract:
Mitochondrial uncoupling protein 3 (UCP3) is consituitively expressed in mitochondria from thymus and
spleen of mice, and confocal microscopy has been used to visualize UCP3 in situ in mouse thymocytes.
UCP3 is present decrease by a half in thymus and a fifth in spleen after three weeks, probably reflecting the
suckling to weaning transition. UCP3 protein levels increase ~3-fold in thymus on starvation, but expression
levels in spleen were unaffected by starvation. Lack of UCP3 had little effect on thymus mass or thymocyte
number . However, lack of UCP3 affected spleen mass and splenocyte number (in the fasted state) and
results in reduced CD4+ single positive cell numbers and reduced double negative cells in the thymus, but as
a 2-fold increase in the proportion of CD4
+
, CD8
+
and DP cells in spleen. Starvation attenuates these
proportionate differences in the spleen. A lack of UCP3 had no apparent effect on basal oxygen consumption
of thymocytes or splenocytes or on oxygen consumption due to mitochondrial proton leak. Splenocytes from
UCP3 knock-out mice are also more resistant to apoptosis than those from wild-type mice. Overall we can
conclude that UCP3 affects thymocyte and spleen cell profiles in the fed and fasted state.
Sponsor
Grant Number
Health Research Board (HRB)
RP/2008/61
Science Foundation Ireland (SFI)
06/IN.1/B67
Author's Homepage:
http://people.tcd.ie/rkporterDescription:
PUBLISHED
Author: Porter, Richard
Type of material:
Journal ArticleCollections:
Series/Report no:
Biochimica et Biophysica Acta (BBA) - Bioenergetics1807
9
Availability:
Full text availableSubject (TCD):
Genes & SocietyLicences: