Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells.
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2008Citation:
Thorpe, S. D., Buckley, C. T., Vinardell, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J., Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells., Biochemical and Biophysical Research Communications, 337, 2, 2008, 458-462Download Item:
Dynamic compression can inhibit chondrogenesis of mesenchymal.pdf (published (author copy) peer-reviewed) 257.9Kb
Abstract:
The objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-?3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5 ? 0.21%w/w vs. 0.94 ? 0.03%w/w) and annulus (1.09 ? 0.09%w/w vs. 0.59 ? 0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue.
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Science Foundation Ireland
Enterprise Ireland
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http://people.tcd.ie/kellyd9http://people.tcd.ie/vinardt
http://people.tcd.ie/vacmpbll
http://people.tcd.ie/cbuckle
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PUBLISHEDPMID: 18851955
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Journal ArticleSeries/Report no:
Biochemical and Biophysical Research Communications337
2
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