Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation
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2021Author:
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Mukhopadhya, A., Santoro, J., O'Driscoll, L., Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation, STAR Protocols, 2021, 2, 100821Download Item:
1-s2.0-S266616672100527X-main.pdf (PDF) 5.825Mb
Abstract:
Separation of highly enriched extracellular vesicles (EVs) fractions from milk is desirable for quantification, cargo analysis, functional characterization, and investigation as delivery vehicles for nutrients and/or therapeutics. However, a
rigorous, reproducible protocol is lacking. This protocol considers a crucial aspect typically overlooked, i.e., that caseins are of similar size to, but more abundant than, EVs in milk. Our protocol combines acid pre-treatment and gradient ultracentrifugation, producing EV-enriched fractions suitable for downstream orthogonal characterization approaches.
For complete details on the use and execution of this protocol, please refer to Mukhopadhya et al. (2021).
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http://people.tcd.ie/mukhopaaDescription:
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Author: Mukhopadhya, Anindya
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Health Sciences, Molecular Biology, Protein Biochemistry, Protein expression and purification, Biotechnology and bioengineeringSubject (TCD):
Cancer , Immunology, Inflammation & Infection , Nanoscience & MaterialsDOI:
https://doi.org/10.1016/j.xpro.2021.100821Licences: