A Unique Polysaccharide containing 3-O-Methylarabinose and 3-O-Methylgalactose from Tinospora sinensis
Citation:
Nagar, S., Hensel, A., Mischnick, P., Kumar, V., A Unique Polysaccharide containing 3-O-Methylarabinose and 3-O-Methylgalactose from Tinospora sinensis, Carbohydrate Polymers, 2018, 193, 326 - 335Abstract:
Tinospora sinensis (Lour.) Merrill is of great therapeutic significance in Indian traditional medicine. Crude polysaccharides were isolated from methanol pre-extracted stems of dried material by successive extractions with cold water, hot water and NaOH (0.25 mol/L) in 0.98, 0.55 and 0.70 % yields respectively. Cold water soluble polysaccharides (CWSP) were purified and fractionated by ion exchange chromatography on DEAE-Sephacel. Neutral polysaccharides were further fractionated on Sepharose CL6B to yield three fractions TW1, TW2, TW3. The study further focuses on structural elucidation of TW1. TW1 was obtained in 0.8 % yield relative to CWSP, with MW of 1.6 × 105 Da. It was composed of 3-O-methyl-arabinose, 3-O-methyl-galactose and galactose in molar ratio of 1.0:6.3:0.9 respectively. Based on per-deuteromethylation, NMR and ESI–MS analyses, TW1 was composed of 1,4-linked 3-O-methyl-β-d-galactopyranose and β-d-galactopyranose backbone with branching at O-6 of 3-O-methyl-β-d-galactosyl residues by 1,5-linked 3-O-methyl-α-l-arabinofuranoside chains. 3-O-methyl-arabinose and 3-O-methyl-galactose have first ever been reported in any polysaccharide and Tinospora genus, respectively.
Author's Homepage:
http://people.tcd.ie/nagarsDescription:
PUBLISHED
Author: Nagar, Shipra
Type of material:
Journal ArticleSeries/Report no:
Carbohydrate Polymers;193;
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Full text availableKeywords:
Tinospora sinensis, 3-O-Me-galactose, 3-O-Me-arabinose, Arabinogalactan, Per-deuteromethylationDOI:
https://doi.org/10.1016/j.carbpol.2018.03.082ISSN:
0144-8617Metadata
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