Multiplex profiling identifies clinically relevant signalling proteins in an isogenic prostate cancer model of radioresistance
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2019Access:
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S Inder, M Bates, N Ni Labhrai, N McDermott, J Schneider, G Erdmann, T Jamerson, AN Flores, A Prina-Mello, P Thirion, PR Manecksha, D Cormican, S Finn, T Lynch, L Marignol, Multiplex profiling identifies clinically relevant signalling proteins in an isogenic prostate cancer model of radioresistance, Scientific Reports, 9, 2019, 1 - 12Download Item:
Inder S_Scientific Reports 2019.pdf (PDF) 1.807Mb
Abstract:
The exact biological mechanism governing the radioresistant phenotype of prostate tumours at a high risk of recurrence despite the delivery of advanced radiotherapy protocols remains unclear. This study analysed the protein expression profiles of a previously generated isogenic 22Rv1 prostate cancer model of radioresistance using DigiWest multiplex protein profiling for a selection of 90 signalling proteins. Comparative analysis of the profiles identified a substantial change in the expression of 43 proteins. Differential PARP-1, AR, p53, Notch-3 and YB-1 protein levels were independently validated using Western Blotting. Pharmacological targeting of these proteins was associated with a mild but significant radiosensitisation effect at 4Gy. This study supports the clinical relevance of isogenic in vitro models of radioresistance and clarifies the molecular radiation response of prostate cancer cells.
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http://people.tcd.ie/batesm1http://people.tcd.ie/prinamea
http://people.tcd.ie/marignl
http://people.tcd.ie/manecksr
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Scientific Reports9
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Prostate cancer, Prostate tumours, RadioresistanceDOI:
http://dx.doi.org/10.1038/s41598-019-53799-7Licences: